Study of regeneration from different tissues or organs of plants is important as it gives information on how a piece of a plant can transform into its whole form. This process is even substantial when we talk about genetic engineering in plants, since no genetic engineering is valuable without knowing first the standard protocol for regenerating the transformed tissue or organs to become a whole plant. This experiment used hypocotyl and cotyledon of tomato cv. Tymoti as the explants was used to study how different concentrations (1.5-3 ppm) of cytokinins (Kinetin (Kin), 6-benzylaminopurine (BAP), thidiazuron (TDZ) and Zeatin (Zn)) affect its growth. As many as 16 explants were used for each treatment. The growth of both explants in the Murashige and Skoog (MS) media + vitamins showed that Zn and TDZ were superior among the other treatments in inducing calli and primordia organ.