This study was aimed to investigate and compare the anthocyanin and phenolic contents between the tuberand callus of Ipomoea batatas (L.). Callus induction was performed in vitro using the Murashige and Skoogmedia supplemented with 0.5 mg/L 2,4-D and 0.1 mg/L kinetin. Meanwhile, the anthocyanin content wasmeasured by pH differential method using cyanidin-3-glucoside as a standard. The total phenolic content wasmeasured using the Folin-Ciocalteu method with gallic acid as a standard. Based on the results obtained, theanthocyanin content of the tuber I. batatas was 1.04 ± 0.12 mg cyanidin-3-glucoside/g fresh weight, while theanthocyanin content of the callus was 0.50 ± 0.07 mg cyanidin-3-glucoside/g fresh weight. The total phenoliccontent of the tuber I. batatas was 0.46 ± 0.01 mg gallic acid/g fresh weight and the phenolic content of thecallus was 0.20 ± 0.01 mg gallic acid/g fresh weight. Both the anthocyanin and phenolic contents of the tuberwere found to be higher than those of the callus of I. batatas.