Rodent Tuber is an anticancer herbal plant from Araceae family which is very sensitive to environmental condition and has a low plantlet reproduction rate. This research was aimed to an obtain effective method of micropropagation on Rodent Tuber plant with high rate multiplication factors. The source of explants used the mother plant originating from Medan (Indonesia). MS medium supplemented with the combination of 0.5 mg/L of BAP and various concentrations of NAA was used. Explants were successfully induced in medium containing 0.5 mg/L of BAP and 0.5 mg/L of NAA. Growing media for plant multiplication were ½ MS and MSO. In the treatment media, BAP was given in five different concentrations, i.e. 0.5, 1, 1.5, 2, and 2.5 mg/L. The result showed that, ½ MS medium added with 1.5 mg/L of BAP was effective in inducing the production of 4.20 ± 1.03 plantlets. In vitro root induction of Rodent Tuber was achieved in MSO medium supplemented with 0.5, 1, 1.5, 2, and 2.5 mg/L of NAA. MSO medium supplemented with 1.5 mg/L of NAA could induce the formation of 43.20 ± 21.21 new roots. Viability percentages of Rodent Tuber from Medan acclimatization was 100%. The usage of MS basal media enriched with BAP and NAA is able to effectively increase the production of new plants and roots of Rodent Tuber plant.