Indonesian native chicken eggshells are an underutilised agricultural waste with potential for conversion into gelatine through sustainable recycling methods. This study evaluated and compared the physicochemical properties of gelatine extracted from Indonesian native chicken eggshell membranes (INCES), broiler chicken eggshell membranes (BCES), and bovine skin. Gelatine was extracted from each source and analysed in triplicate. Structural characteristics and elemental composition were investigated using Scanning Electron Microscopy combined with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier Transform Infrared Spectroscopy (FTIR), and X-ray Fluorescence (XRF) were used to elucidate structural features and elemental composition differences among the samples. The SEM photos show that BCES gelatine had larger, more irregular structures, while INCES gelatine had finer, more spherical particles. Bovine gelatine displayed a smoother, fibrous morphology. Nine significant elements were found by EDX elemental analysis (C, N, O, Na, Mg, Al, Si, Ca, and Au). While carbon elements predominated in bovine gelatine, calcium was present in larger amounts in eggshell protein (INCES & BCES). Typical amide bands (A, B, I, II, and III) were visible in the FTIR spectra. In contrast to BCES gelatine, which tended to exhibit more random coil properties, the spectra of ICES and bovine gelatine revealed β-sheet structures. Analysis using XRF revealed that INCES gelatine contained elevated calcium levels, most likely due to incomplete demineralisation during extraction. Overall, INCES represents a promising alternative gelatine source that supports the valorisation of poultry by-products. These findings demonstrate that INCES gelatine exhibits a distinctive mineral-rich, β-sheet-dominant profile relative to bovine gelatine, positioning it as a promising, halal-compliant alternative for functional food and nutraceutical applications, while further functional and safety validation is required before any biomedical application.