Muscle activation in baseball swing is a subject which has been extensively studied compared to softball swing. However, muscle activation in baseball swing should not be generalised to softball swing due to differences between these sports in terms of ball size, speed, and the angle of pitch. This study aims to identify major muscle activations during different phases in softball batting, namely loading, contact, and follow through. Ten female softball players participated in twenty trials of hitting a stationary ball. The peak normalized sEMG for all muscles during softball batting phases was analysed and recorded as the percentage of maximum voluntary contraction (% of MVC). During stance, it was found that left pectoralis major involved the highest muscle activity with 9.08% of MVC, while the right rectus abdominus involved the least muscle activity with 1.43% of MVC. During the loading phase, the right pectoralis major involved the highest muscle activity with 22.8% of MVC, while the right middle deltoid involved the least muscle activity with 7.03% of MVC. During the contact phase, the highest muscle activity was observed from the right external oblique with 23.93% of MVC, while the least muscle activity with 7.98% of MVC was observed from the right biceps femoris. During the follow through phase, left gastrocnemius involved the highest muscle activity with 18.09% of MVC, while right posterior deltoid had the least muscle activity with 4.49% of MVC. Overall, although nearly the same muscles were involved in softball and baseball swing, different activation patterns were shown by several muscle groups during softball swing. These findings would be useful for coaches to develop training programmes which specifically aim for the improvement in softball batting performance.