e-ISSN 2231-8542
ISSN 1511-3701

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Quantification of Gallic Acid and Tannic Acid from Quercus infectoria (Manjakani) and their Effects on Antioxidant and Antibacterial Activities

Ab. Rahman, N. S., Md. Salleh L., Abd. Majid F. A. and Harisun, Y.

Pertanika Journal of Tropical Agricultural Science, Volume 23, Issue 2, July 2015

Keywords: Bioactive compound, solvent, antioxidant activity, antibacterial activity, gallic acid, HPLC, tannic acid, and Q. infectoria.

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Effects of different types of solvent on the antioxidant and antibacterial activity of Quercus infectoria extract have not been well documented. Therefore, extraction process was conducted using conventional Soxhlet extraction with six different types of solvent (100% methanol, ethanol, acetone, water and 70% methanol, and ethanol). High performance liquid chromatography was implemented to identify gallic acid and tannic acid in the extracts. Water extracts contained the highest concentration of both gallic acid and tannic acid compared to other types of solvent; 51.14 mg/g sample and 1332.88 mg/g sample of gallic acid and tannic acid. Meanwhile, antioxidant and antibacterial activity were tested using DPPH free radicals scavenging and disc diffusion assay. Results demonstrated that water extracts gave the highest antioxidant activity (approximately 94.55%), while acetone extract gave the largest inhibition zone for disc diffusion assay (19.00mm respectively). The results also revealed rich sources of gallic acid and tannic acid in Q. infectoria which might provide a novel source of these natural antioxidant and antibacterial activity.

ISSN 1511-3701

e-ISSN 2231-8542

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