e-ISSN 2231-8542
ISSN 1511-3701

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MRN Complex and ATM Kinase Inhibitors Impacts towards UVC-Treated Zebrafish Embryonic Development

Shazrul Fazry, Malina Kumaran, Nahid Khalili, Ibrahim Mahmood, Herryawan Ryadi Eziwar Dyari, Nur Hidayah Jamar, Douglas Law, Nik Marzuki Sidik, Nor Azizan Abdul Murad and Yi Chieh Lim

Pertanika Journal of Tropical Agricultural Science, Volume 27, Issue 4, October 2019

Keywords: ATM Kinase, Danio rerio, DNA damage, MRN complex, zebrafish

Published on: 21 October 2019

The zebrafish (Danio rerio) has become a prevailing vertebrate model for developmental biology studies due to its ease of care, rapid embryogenesis stages development and translucent embryos. In this studies, ATM Kinase and MRN complex role as DNA damage response proteins during embryogenesis was examined by using specific MRN complex (Mirin) and ATM Kinase inhibitors (Ku60019 and Ku55933). To create DNA lesions in zebrafish, embryos at mid-blastula transition (MBT) stage were exposed to inhibitors (Mirin, Ku60019 or Ku55933) and later exposed to UVC irradiation wavelength of between 100 to 280 nm. Hatching but with visible physical deformation was observed for embryos treated with Mirin, Ku60019 or Ku55933 and UVC exposure at concentration of 3µM, 1.5 nM and 3nM or lower, respectively up to 72 hours-post fertilisation (hpf). On the other hand, no deformities were observed for all control as well as mock treated embryos. This study confirmed that DNA damage response proteins are crucial during embryo development to prevent undesired abnormal biological development. Thus, it is proven that protein inhibitors are a cheaper alternative in valuating specific protein roles during embryogenesis compared to both genomic and transcription modification tools.

ISSN 1511-3701

e-ISSN 2231-8542

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