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Effect of Pre-Treatment Methods on the Extractability of Christia vespertilionis by Supercritical Carbon Dioxide

Izni Atikah Abd Hamid, Najla Laazizi, Ana Najwa Mustapa and Norazah Abd Rahman

Pertanika Journal of Tropical Agricultural Science, Volume 31, Issue 5, August 2023


Keywords: Carbon dioxide, Christia vespertilionis, co-solvent, medicinal compounds, pre-treatment, supercritical extraction

Published on: 31 July 2023

Christia vespertilionis is a medicinal herb traditionally used as a complementary and alternative medicine to treat cancer and malaria. This study investigated the effect of pre-treatments of the Christia vespertilionis plant on supercritical CO2 extraction yield and solubility. Four pre-treatments were studied: drying and grinding, doping with absolute ethanol (99%) and 80% (v/v) of ethanol/water, and microwave pre-treatment. The supercritical CO2 extraction was conducted at a constant 13.8 MPa, 40℃ with 24 mL/min flow rate in 40 min of extraction time. It was found that the dried sample after drying and grinding pre-treatment produced the highest yield of 4.56 mg/g, whereas the lowest yield was obtained for the fresh leaves’ samples treated with microwave irradiation (1.26 mg/g). Doping techniques with absolute ethanol and 80% (v/v) were comparable in the 2.64 to 2.94 mg/g. GCMS results revealed that Christia vespertilionis extract comprises antioxidants, mainly phytol, limonene, and other medicinal compounds such as α-monolaurin and l-ascorbyl 2,6-dipalmitate. This study indicates that adding co-solvent was not the primary technique in supercritical CO2 extraction to increase the extractability of compounds of interest from plant matrices.

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