PERTANIKA JOURNAL OF TROPICAL AGRICULTURAL SCIENCE

 

e-ISSN 2231-8542
ISSN 1511-3701

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Optimization of Headspace Solid Phase Microextraction (HS-SPME) for the Extraction of Volatile Organic Compounds (VOCs) in Mangoes (Harumanis cv.) Using 2 Stages Multivariate Analysis

Siti Raihan Zakaria, Ruziyati Tajuddin, Rozita Osman, Norashikin Saim and Mardiana Saaid

Pertanika Journal of Tropical Agricultural Science, Volume 25, Issue S, March 2017

Keywords: CCD, Harumanis, HS-SPME, VOCs

Published on: 05 Dec 2017

Headspace solid phase microextraction (HS-SPME) was used to isolate volatile compounds (VOCs) from mangoes (Harumanis cv.). Among the four SPME fibres investigated, the mixed phase coating, 65 µm polydimethyl siloxane-divinylbenzene (DVB/PDMS) showed the highest efficiency in extracting VOCs as 26 compounds were detected with the total area of 9.6 x 109. The optimization of SPME factors was conducted in 2 stages using multivariate design. The first stage involved screening of the significant factors using the Plackett-Burman (P-B) design, followed by the optimization of the significant factors utilizing Central Composite Design (CCD). The experimental design for both P-B and CCD design was generated using Design-Expert version 6.0.4 (Stat Ease Software). Extraction time and temperature appeared to be the most significant factors in extracting VOCs in mangoes, with the optimum conditions prevailing at 55°C and 34 minutes respectively.

ISSN 1511-3701

e-ISSN 2231-8542

Article ID

JST-S0167-2016

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