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Survival Rate and Growth Performance of Holothuria scabra Towards Different Stocking Densities and Feeding with Spirulina

Syed Mohamad Azim Syed Mahiyuddin, Muhammad Asyraf Abd Latip, Zainuddin Ilias, Khairudin Ghazali and Nik Daud Nik Sin

Pertanika Journal of Tropical Agricultural Science, Volume 47, Issue 1, February 2024

DOI: https://doi.org/10.47836/pjtas.47.1.14

Keywords: Holothuria scabra, sandfish, sea cucumber, Spirulina, stocking density

Published on: 23 Febuary 2024

Holothuria scabra is widely used in traditional medicine or consumed as a healthy or exotic food, causing wild stocks to decrease. Therefore, aquaculture is the best solution to address this issue and support the growing market demand. However, up to now, H. scabra production is facing problems related to the nursery phase, survival rate of larvae and juveniles, production cost, and broodstock for mass production. In this study, the juveniles of H. scabra were divided into three different stocking densities and fed with 1 g of dissolved Spirulina powder once on alternate days. Their length was recorded every two weeks. After six weeks, the juveniles reared with 100 individual densities showed the highest survival rate at 80% compared to 200 and 400 stocking densities. Meanwhile, every tank showed a positive growth rate, indicating that Spirulina powder could potentially promote the growth of juvenile H. scabra. The specific growth rate for stocking density at 100, 200, and 400 were 1.2, 0.86, and 1.37%/day, respectively. In conclusion, the optimum initial stocking density is between 100 and 200 individuals for a 1-ton fibreglass tank with a 500 L water capacity. The Spirulina can be used as the main protein source as compared to other diets for juvenile H. scabra.

  • Abdelaty, M., Al-Solami, L., Al-Harbi, M., & El-Regal, M. A. (2021). Utilization of different types of microalgae to improve hatcheries production of the sea cucumber Holothuria scabra Jaeger, 1833 in the Red Sea, Egypt. Egyptian Journal of Aquatic Biology and Fisheries, 25(2), 193-204. https://doi.org/10.21608/ejabf.2021.161824

  • Abidin, N. A., Muhamad Shaleh, S. R., Ching, F. F., Othman, R., Manjaji-Matsumoto, M., Mustafa, S., & Senoo, S. (2019). Appropriate diet and stocking density for sea cucumber (Holothuria scabra) larvae rearing. In IOP Conference Series: Journal of Physics (Vol. 1358, No. 1, p. 012015). IOP Publishing. https://doi.org/10.1088/1742-6596/1358/1/012015

  • Altamirano, J. P., & Noran-Baylon, R. D. (2020). Nursery culture of sandfish Holothuria scabra in sea-based floating hapa nets: Effects of initial stocking density, size grading and net replacement frequency. Aquaculture, 526, 735379. https://doi.org/10.1016/j.aquaculture.2020.735379

  • Altamirano, J. P., Recente, C. P., & Rodriguez, J. C. (2017). Substrate preference for burying and feeding of sandfish Holothuria scabra juveniles. Fisheries Research, 186(Part 2), 514-523. https://doi.org/10.1016/j.fishres.2016.08.011

  • Ardiansyah, A., Rasyid, A., Siahaan, E. A., Pangetistu, R., & Murniasih, T. (2020). Nutritional value and heavy metals content of sea cucumber Holothuria scabra commercially harvested in Indonesia. Current Research in Nutrition and Food Science Journal, 8(3), 765-773. https://doi.org/10.12944/CRNFSJ.8.3.09

  • Asha, P. S., & Diwakar, K. (2013). Effect of stocking density on the hatching rate, larval and early juvenile rearing of edible sea cucumber Holothuria scabra (Jaeger, 1883). Indian Journal of Geo-Marine Sciences, 42(2), 191-195.

  • Asha, P. S., Rajagopalan, M., & Diwakar, K. (2004). Effect of sea weed, sea grass and powdered algae in rearing the hatchery produced juveniles of Holothuria (metriatyla) scabra, Jaeger. http://eprints.cmfri.org.in/6228/1/610.pdf

  • Barclay, K., Voyer, M., Mazur, N., Payne, A. M., Mauli, S., Kinch, J., Fabinyi, M., & Smith, G. (2017). The importance of qualitative social research for effective fisheries management. Fisheries Research, 186(Part 2), 426-438. https://doi.org/10.1016/j.fishres.2016.08.007

  • Battaglene, S. C., & Bell, J. D. (2004). The restocking of sea cucumbers in the Pacific Islands. In D. M. Bartley & K. M. Leber (Eds.), Marine ranching (pp. 109-132). Food and Agriculture Organization.

  • Battaglene, S. C., Seymour, J. E., & Ramofafia, C. (1999). Survival and growth of cultured juvenile sea cucumbers, Holothuria scabra. Aquaculture, 178(3-4), 293-322. https://doi.org/10.1016/S0044-8486(99)00130-1

  • Cantero, Q., Nair, M., & Smith, J. (2016). Effect of stocking density on the growth , survival, and settlement of sandfish sea cucumber (Holothuria scabra). Journal of Health Disparities Research and Practice, 9(5), 31.

  • Dabbagh, A.-R., Mohammadikia, D., & Keshavarz, M. (2012). Feeding some commercial diets in the captive breeding of the sea cucumber, Holothuria scabra in Iran. Annals of Biological Research, 3(2), 1050-1053.

  • Gorospe, J. C., Altamirano, J. P., & Juinio‐Meñez, M. A. (2017). Viability of a bottom‐set tray ocean nursery system for Holothuria scabra Jaeger 1833. Aquaculture Research, 48(12), 5984-5992. https://doi.org/10.1111/are.13423

  • Han, Q., Keesing, J. K., & Liu, D. (2016). A review of sea cucumber aquaculture, ranching, and stock enhancement in China. Reviews in Fisheries Science & Aquaculture, 24(4), 326-341. https://doi.org/10.1080/23308249.2016.1193472

  • Indriana, L. F., Firdaus, M., & Munandar, H. (2017). Survival rate and growth of juvenile sandfish (Holothuria scabra) in various rearing conditions. Marine Research in Indonesia, 42(1), 11-18. https://doi.org/10.14203/MRI.V41I2.156

  • Kamarudin, K. R., Rehan, M. M., & Rehan, A. M. (2019). Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes. Maritime Technology and Research, 1(2), 28-44. https://doi.org/10.33175/mtr.2019.146259

  • Lavitra, T., Rasolofonirina, R., & Eeckhaut, I. (2010). The effect of sediment quality and stocking density on survival and growth of the sea cucumber Holothuria scabra reared in nursery ponds and sea pens. Western Indian Ocean Journal of Marine Science, 9(2), 153-164.

  • Lavitra, T., Rasolofonirina, R., Grosjean, P., Jangoux, M., & Eeckhaut, I. (2009). The effect of food quality and rearing density on the growth and survival of epibenthic juveniles of the sea cucumber Holothuria scabra. Western Indian Ocean Journal of Marine Science, 8(1), 87-95. https://doi.org/10.4314/wiojms.v8i1.56678

  • Magcanta, M. L. M., Sornito, M. B., Espadero, A. D. A., Bacosa, H. P., & Uy, W. H. (2021). Growth, survival, and behavior of early juvenile sandfish Holothuria scabra (Jaeger, 1883) in response to feed types and salinity levels under laboratory conditions. Philippine Journal of Science, 150(5), 871-884.

  • Militz, T. A., Leini, E., Duy, N. D. Q., & Southgate, P. C. (2018). Successful large-scale hatchery culture of sandfish (Holothuria scabra) using micro-algae concentrates as a larval food source. Aquaculture Reports, 9, 25-30. https://doi.org/10.1016/j.aqrep.2017.11.005

  • Purcell, S. W. (2004). Criteria for release strategies and evaluating the restocking of sea cucumbers. In A. Lovatelli, C. Conand, S. W. Purcell, S. Uthicke, J.-F. Hamel, & A. Mercier (Eds.), Advances in sea cucumber aquaculture and management (pp. 181-191). Food and Agriculture Organization.

  • Ridwanudin, A., Indriana, L., & Kunzmann, A. (2018). No difference in nutritional profiles of wild and cultured juvenile sandfish, Holothuria scabra. Annual Research and Review in Biology, 26(5), 1-11. https://doi.org/10.9734/arrb/2018/41457

  • Sibonga, R. C., Laureta, L. V., Lebata-Ramos, M. J. H., Nievales, M. F. J., & Pedroso, F. L. (2022). Comparison of commercial Spirulina, live Navicula sp. and Chaetoceros calcitrans concentrate as settlement cues for sandfish, Holothuria scabra. Journal of Applied Phycology, 34, 2099-2105. https://doi.org/10.1007/s10811-022-02767-2

  • Simoes, M., & Knauer, J. (2012). Marking juvenile sandfish, Holothuria scabra, with the fluorochrome oxytetracycline - A preliminary report. SPC Bêche-de-mer Information Bulletin, 32, 3-5.

  • Sitoresmi, D. A. N., & Pursetyo, K. T. (2020). The effect of concrete tanks for the breeding technique of the sand sea cucumber (Holothuria scabra). In IOP Conference Series: Earth and Environmental Science (Vol. 441, No. 1, p. 012054). IOP Publishing. https://doi.org/10.1088/1755-1315/441/1/012054

  • Wu, B., Xia, S., Rahman, M. M., Rajkumar, M., Fu, Z., Tan, J., & Yang, A. (2015). Substituting seaweed with corn leaf in diet of sea cucumber (Apostichopus japonicus): Effects on growth, feed conversion ratio and feed digestibility. Aquaculture, 444, 88-92. https://doi.org/10.1016/j.aquaculture.2015.03.026

  • Yuniati, R., & Sulardiono, B. (2020). Effectivity of Holothuria scabra and Spirulina platensis extract combination as an antiinflammatory agent measured by carrageenan-induced rat paw edema. ILMU KELAUTAN: Indonesian Journal of Marine Sciences, 25(3), 103-109. https://doi.org/10.14710/ik.ijms.25.3.103-109

  • Yussuf, Y. S., & Yahya, S. A. (2021). Stocking density, growth and survival rate of post-settled juveniles of Holothuria scabra (Jaeger 1833) reared in an ocean-based floating hapa. Tanzania Journal of Science, 47(3), 1041-1054. https://doi.org/10.4314/tjs.v47i3.15

ISSN 1511-3701

e-ISSN 2231-8542

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