Home / Regular Issue / JTAS Vol. 47 (1) Feb. 2024 / JTAS-2795-2023

 

Effects of Coconut Water and Banana Homogenate on Shoot Regeneration of Meyer Lemon (Citrus × meyeri)

Stephanie Qiao Er Wong, Najwa Amalina Haradzi, Dahmendra Sriskanda, Sreeramanan Subramaniam and Bee Lynn Chew

Pertanika Journal of Tropical Agricultural Science, Volume 47, Issue 1, February 2024

DOI: https://doi.org/10.47836/pjtas.47.1.11

Keywords: Banana homogenate, Citrus × meyeri, coconut water, Meyer lemon, organic additive, shoot regeneration

Published on: 23 Febuary 2024

Meyer lemon (Citrus × meyeri) is a hybrid citrus fruit from the Rutaceae family, originating from China. It is well-known for its distinctive appearance and flavor, as well as its health-nourishing nutrients. Micropropagation is an efficient alternative in the multiplication of plant stocks suitable for the commercial scale. The inclusion of organic additives in culture media has been found to provide a cost-effective option as a plant growth stimulant for in vitro plant development. The current study intends to assess the effects of coconut water and banana homogenate in the regeneration of Meyer lemon. In vitro, shoots were treated in half-strength Murashige and Skoog media fortified with 2 mg/L 6-benzylaminopurine with varying concentrations of coconut water and banana homogenate without sucrose. Results revealed that the treatment of 30% coconut water and 40 g/L banana homogenate resulted in the greatest proliferation of new shoots (3.00 ± 0.873 and 1.57 ± 0.297, respectively), whereas treatment of 40% coconut water resulted in the greatest shoot elongation of 0.239 ± 0.026 cm. The current study suggested the incorporation of coconut water and banana homogenate as potential substitutes for carbon sources and growth stimulants in the regeneration of Meyer lemon.

  • Adubofuor, J., Amoah, I., Batsa, V., Agyekum, P. B., & Buah, J. A. (2016). Nutrient composition and sensory evaluation of ripe banana slices and bread prepared from ripe banana and wheat composite flours. American Journal of Food and Nutrition, 4(4), 103-111. https://doi.org/10.12691/ajfn-4-4-3

  • Ahloowalia, B. S., Prakash, J., Savangikar, V. A., & Savangikar, C. (2002). Plant tissue culture. In Proceedings of A Technical Meeting (pp. 3-10). International Atomic Energy Agency. https://www-pub.iaea.org/mtcd/publications/pdf/te_1384_web.pdf

  • Al-Khateeb, A. A. (2008). Regulation of in vitro bud formation of date palm (Phoenix dactylifera L.) cv. Khanezi by different carbon sources. Bioresource Technology, 99(14), 6550-6555. https://doi.org/10.1016/j.biortech.2007.11.070

  • Al-Khayri, J. M. (2010). Somatic embryogenesis of date palm (Phoenix dactylifera L.) improved by coconut water. Biotechnology, 9(4), 477-484. https://doi.org/10.3923/biotech.2010.477.484

  • Apensa, V., & Mastuti, R. (2018). Effect of banana homogenate on shoot regeneration of ciplukan (Physalis angulata L.). The Journal of Experimental Life Science, 8(1), 53-60. https://doi.org/10.21776/ub.jels.2018.008.01.09

  • Aurore, G., Parfait, B., & Fahrasmane, L. (2009). Bananas, raw materials for making processed food products. Trends in Food Science and Technology, 20(2), 78-91. https://doi.org/10.1016/j.tifs.2008.10.003

  • Badrelden, A. (2017). Establishment of in direct propagation of mandarin (Citrus reticulata L.) using tissue culture. Egyptian Journal of Botany, 57(3), 405-416. https://doi.org/10.21608/ejbo.2017.799.1050

  • Carimi, F., & De Pasquale, F. (2003). Micropropagation of Citrus. In S. M. Jain & K. Ishii (Eds.), Micropropagation of woody trees and fruits (Vol. 75, pp. 589-619). Springer. https://doi.org/10.1007/978-94-010-0125-0_20

  • Chambial, S., Dwivedi, S., Shukla, K. K., John, P. J., & Sharma, P. (2013). Vitamin C in disease prevention and cure: An overview. Indian Journal of Clinical Biochemistry, 28, 314-328. https://doi.org/10.1007/s12291-013-0375-3

  • Daud, N., Taha, R. M., Md, L., & Alimon, H. (2011). Effects of different organic additives on in vitro shoot regeneration of Celosia sp. Pakistan Journal of Biological Sciences, 14(9), 546–551. https://doi.org/10.3923/pjbs.2011.546.551

  • D’Elia, L., Barba, G., Cappuccio, F. P., & Strazzullo, P. (2011). Potassium intake, stroke, and cardiovascular disease: A meta-analysis of prospective studies. Rational Pharmacotherapy in Cardiology, 7(3), 371-381. https://doi.org/10.20996/1819-6446-2011-7-3-371-381

  • Ge, L., Yong, J. W. H., Goh, N. K., Chia, L. S., Tan, S. N., & Ong, E. S. (2005). Identification of kinetin and kinetin riboside in coconut (Cocos nucifera L.) water using a combined approach of liquid chromatography-tandem mass spectrometry, high performance liquid chromatography and capillary electrophoresis. Journal of Chromatography B, 829(1-2), 26-34. https://doi.org/10.1016/j.jchromb.2005.09.026

  • George, E. F., Hall, M. A., & Klerk, G. J.-D. (Eds.). (2008). Plant propagation by tissue culture: Volume 1. The background. Springer. https://doi.org/10.1007/978-1-4020-5005-3

  • Gmitter, F. G., & Hu, X. (1990). The possible role of Yunnan, China, in the origin of contemporary Citrus species (Rutaceae). Economic Botany, 44, 267-277. https://doi.org/10.1007/bf02860491

  • Goble, T. A., Dames, J. F., Hill, M. P., & Moore, S. D. (2011). Investigation of native isolates of entomopathogenic fungi for the biological control of three citrus pests. Biocontrol Science and Technology, 21(10), 1193-1211. https://doi.org/10.1080/09583157.2011.608907

  • González-Molina, E., Domínguez-Perles, R., Moreno, D. A., & García-Viguera, C. (2010). Natural bioactive compounds of Citrus limon for food and health. Journal of Pharmaceutical and Biomedical Analysis, 51(2), 327-345. https://doi.org/10.1016/j.jpba.2009.07.027

  • Haradzi, N. A., Khor, S. P., Subramaniam, S., & Chew, B. L. (2021). Regeneration and micropropagation of Meyer lemon (Citrus × meyeri) supported by polymorphism analysis via molecular markers. Scientia Horticulturae, 286, 110225. https://doi.org/10.1016/j.scienta.2021.110225

  • Hardy, S. (2004). Growing lemons in Australia - A production manual. NSW Department of Primary Industries.

  • Islam, M. O., Islam, M. S., & Saleh, M. A. (2015). Effect of banana extract on growth and development of protocorm like bodies in Dendrobium sp. orchid. The Agriculturists, 13(1), 101-108. https://doi.org/10.3329/agric.v13i1.26553

  • Kaur, S., & Bhutani, K. K. (2012). Organic growth supplement stimulants for in vitro multiplication of Cymbidium pendulum (Roxb.) Sw. Horticultural Science, 39(1), 47-52. https://doi.org/10.17221/52/2011-hortsci

  • Khatun, M., Roy, P. K., & Razzak, M. A. (2018). Additive effect of coconut water with various hormones on in vitro regeneration of carnation (Dianthus caryophyllus L.). Journal of Animal and Plant Sciences, 28(2), 589-596.

  • Ladaniya, M. S. (2008). Citrus fruit: Biology, technology and evaluation. Academic Press. https://doi.org/10.1016/B978-0-12-374130-1.X5001-3

  • Lee, H.-S., Kim, Y.-B., Seo, C., Ji, M., Min, J., Choi, S., Kim, H.-B., Park, H.-J., Lee, G., Lee, W. J., & Paik, M.-J. (2019). Characterization of ripening bananas by monitoring the amino acid composition by gas chromatography-mass spectrometry with selected ion monitoring and star pattern analysis. Analytical Letters, 52(16), 2496-2505. https://doi.org/10.1080/00032719.2019.1615076

  • Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

  • Pacheco, G., Garcia, R., Lugato, D., Vianna, M., & Mansur, E. (2012). Plant regeneration, callus induction and establishment of cell suspension cultures of Passiflora alata Curtis. Scientia Horticulturae, 144, 42-47. https://doi.org/10.1016/j.scienta.2012.06.022

  • Peña, L., Cervera, M., Fagoaga, C., Romero, J., Ballester, A., Soler, N., Pons, E., Rodríguez, A., Peris, J., Juárez, J., & Navarro, L. (2009). Citrus: Part 5. Transgenic tropical and subtropical fruits and nuts. In C. Kole & T. C. Hall (Eds.), Compendium of transgenic crop plants (pp. 1-62). Blackwell Publishing. https://doi.org/10.1002/9781405181099.k0501

  • Penniston, K. L., Steele, T. H., & Nakada, S. Y. (2007). Lemonade therapy increases urinary citrate and urine volumes in patients with recurrent calcium oxalate stone formation. Urology, 70(5), 856-860. https://doi.org/10.1016/j.urology.2007.06.1115

  • Prades, A., Dornier, M., Diop, N., & Pain, J.-P. (2012). Coconut water uses, composition and properties: A review. Fruits, 67, 87-107. https://doi.org/10.1051/fruits/2012002

  • Rahman, M. S., Mouri, N. J., Nandi, N. C., Akter, S., & Khan, M. S. (2018). In vitro micropropagation of Jasminum grandiflorum L. Bangladesh Journal of Scientific and Industrial Research, 53(4), 277-282. https://doi.org/10.3329/bjsir.v53i4.39191

  • Rao, N., Sharma, M., & Sharma, A. (2016). Development of products rich in dietary fiber and antioxidant prepared from lemon peel. Global Journal of Biology, Agriculture and Health Sciences, 5(2), 120-123.

  • Rattanpal, H. S., Kaur, G., & Gupta, M. (2011). In vitro plant regeneration in rough lemon (Citrus jambhiri Lush) by direct organogenesis. African Journal of Biotechnology, 10(63), 13724-13728. https://doi.org/10.5897/ajb09.1264

  • Sandoval Prando, M. A. S., Chiavazza, P., Faggio, A., & Contessa, C. (2014). Effect of coconut water and growth regulator supplements on in vitro propagation of Corylus avellana L. Scientia Horticulturae, 171, 91-94. https://doi.org/10.1016/j.scienta.2014.03.052

  • Takahashi, K., & Kinoshita, T. (2016). The regulation of plant cell expansion: Auxin-induced turgor-driven cell elongation. In R. J. Rose (Ed.). Molecular cell biology of the growth and differentiation of plant cells (pp. 156-173). CRC Press. https://doi.org/10.1201/b20316-13

  • Tanaka, T., & Tanaka, C. (1954). Species problem in citrus: A critical study of wild and cultivated units of citrus, based upon field studies in their native homes. Japanese Society for the Promotion of Science.

  • United States Department of Agriculture. (2021). Citrus: World markets and trade. USDA. https://downloads.usda.library.cornell.edu/usda-esmis/files/w66343603/b8516k98g/2514ph35q/citrus.pdf

  • Vilcherrez-Atoche, J. A., Rojas-Idrogo, C., & Delgado-Paredes, G. E. (2020). Micropropagation of Cattleya maxima J. Lindley in culture medium with banana flour and coconut water. International Journal of Plant, Animal and Environmental Sciences, 10(4), 179-193.

  • Wyman, H., & Palmer, J. K. (1964). Organic acids in the ripening banana fruit. Plant Physiology, 39(4), 630-633. https://doi.org/10.1104/pp.39.4.630

  • Xu, X., Du, X., Wang, F., Sha, J., Chen, Q., Tian, G., Zhu, Z., Ge, S., & Jiang, Y. (2020). Effects of potassium levels on plant growth, accumulation and distribution of carbon, and nitrate metabolism in apple dwarf rootstock seedlings. Frontiers in Plant Science, 11, 904. https://doi.org/10.3389/fpls.2020.00904

  • Ye, X. (Ed.) (2018). Phytochemicals in citrus: Applications in functional foods (1st ed.). CRC Press. https://doi.org/10.1201/9781315369068

  • Yong, J. W. H., Ge, L., Ng, Y. F., & Tan, S. N. (2009). The chemical composition and biological properties of coconut (Cocos nucifera L.) water. Molecules, 14(12), 5144-5164. https://doi.org/10.3390/molecules14125144

  • Zaffari, G. R., Kerbauy, G. B., Kraus, J. E., & Romano, E. C. (2000). Hormonal and histological studies related to in vitro banana bud formation. Plant Cell, Tissue and Organ Culture, 63, 187-192. https://doi.org/10.1023/A:1010611318932