e-ISSN 2231-8542
ISSN 1511-3701

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Enzymatic Dehairing of Goat Skin Using Keratinase from Bacillus sp. MD24, A Newly Isolated Soil Bacterium

Suharti Suharti, Maurilla Trisna Riesmi, Arina Hidayati, Umi Faridatuz Zuhriyah, Surjani Wonorahardjo and Evi Susanti

Pertanika Journal of Tropical Agricultural Science, Volume 41, Issue 3, August 2018

Keywords: Bacillus, dehairing, keratinase, dehaired goat skin, keratin-degrading bacterium

Published on: 30 Aug 2018

A newly keratin-degrading bacterium was isolated from a residential soil at Wajak district, Malang, East Java, Indonesia. Sequence homology analysis showed the 16S rRNA had only 89% sequence identity to the available bacterial 16S rRNA which led to a discovery of a new species of Bacillus. The bacterium was named Bacillus sp MD24. The isolated bacterium degraded 71% mass of whole chicken feathers within 10 days. Keratinase fermentation using 1% of chicken feathers as sole source of carbon and nitrogen exhibited highest enzyme activity at third day under optimum condition (pH of 8 and temperature of 37oC). At enzyme concentration of 0.3 U/mL, the crude extract keratinase started to exhibit dehairing activity on goat skin after an overnight incubation; the best incubation was achieved at 72 hours. Surface of enzymatically dehaired goat skin was compared to chemically dehaired skin goat. The result showed similar or improved surface of the skin which makes the crude keratinase from Bacillus sp MD24 a potential candidate for application in leather industry to avoid pollution problems due to the use of chemicals.

ISSN 1511-3701

e-ISSN 2231-8542

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