Escalating pressures along with organisational change in universities has led to the increased importance of research on work-life balance, well-being and organisational climate amongst university staff. Grounded in Work/Family Border Theory and Social Exchange Theory, this study investigated the causal relationships between work-life balance and well-being within the context of Open Distance Learning (ODL) University in Malaysia. This study also introduced organisational climate as potential moderator to investigate the indirect relationship on work-life balance and well-being. Through the Partial Least Square (PLS) analysis on 94 respondents, findings suggested that work-life balance for staff only impact on their psychological well-being. In addition, contrary to our expectations, the results revealed that organisational climate did not moderate the relationship between work-life balance and individual well-being. Several recommendations that will be significant to improve career satisfaction, and to have more positives work environment are discussed.