e-ISSN 2231-8542
ISSN 1511-3701

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In-vitro Antidermatophytic Activity of Methanolic Fractions from Entada spiralis Ridl. Stem Bark and Its Bioautographic Profile

Aiza Harun, Siti Zaiton Mat So’ad, Norazian Mohd Hassan and Neni Kartini Che Mohd Ramli

Pertanika Journal of Tropical Agricultural Science, Volume 22, Issue 1, January 2014

Keywords: Leguminoceae, Entada spiralis antidermatophytic activity, dermatophytes, bioautography

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This study was performed to evaluate the antifungal activities of methanolic fractions from the stem bark of Entada spiralis Ridl. against human dermatophytes and yeast-like fungus in vitro. Three types of human dermatophyte, Trichophyton mentagrophytes ATCC 9533, Microsporum gypseum ATCC 24102 and Trichophyton tonsurans ATCC 28942, and one yeast-like fungus, Candida glabrata ATCC 66032, were tested against the methanolic fractions labelled FA1, FA4 and FA5. T. mentagrophytes, T. tonsuran and M. gypseum were susceptible to all tested fractions in a concentration-dependent manner whereas C. glabrata was resistant. Fraction FA1 at a concentration of 400 mg/mL was found to exhibit the highest antifungal activity with the inhibition zone diameter of 22 mm (T. mentagrophytes). This fraction showed a minimum inhibitory concentration MIC of 0.097 mg/mL while the MIC value for the fraction FA4 and fraction FA5 was 3.12 mg/ml and 1.56 mg/ml respectively. Agar overlay bioautography assay results showed that most of the bioactive compounds were found in the fraction FA1. Based on these findings, it can be concluded that the stem bark extracts of E. spiralis can be a future source of potent natural antimicrobial drugs for superficial skin diseases.

ISSN 1511-3701

e-ISSN 2231-8542

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