e-ISSN 2231-8542
ISSN 1511-3701

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Random Amplified Polymorphism DNA Method to Authenticate Indonesian medicinal Plant Ciplukan (Physalis angulata;Solanaceae)

Topik Hidayat, Lea Juliana Yosnata, Linda Tri Wulandari, Didik Priyandoko and Siti Aisyah

Pertanika Journal of Tropical Agricultural Science, Volume 25, Issue S, November 2017

Keywords: Ashwaganda, Ciplukan, Polymorphism, RAPD fingerprinting

Published on: 04 Jan 2018

Ciplukan (Physalis angulata) is a medicinal plant in Indonesia, belonging to the family of Solanaceae. Based on molecular phylogenetic analysis, this plant is relative to Ashwaganda (Withania somnifera), the famed South Asia medicinal plant touted to kill cancer cells. A study was conducted on genetic variation of Ciplukan using Random Amplified Polymorphic DNA (RAPD) marker. A total of 23 plants from Northern, Southern, Central, Eastern, and Western part of Bandung were examined The RAPD analyses were performed using three selected random primers (OPA1, OPB17, and OPB10). Clustering analysis was conducted based on Unweighted Pair-Group Method with Arithmetic Average (UPGMA) using MEGA 4. Dendrogram showed that the sample plants were not grouped by their geographic localities, suggesting that a genetic interaction occurs among plants from five different locations. This result was also supported by the high level of estimated gene flow (Nm= 1.0919). It is likely due to the nature of self-incompatibility in Ciplukan which requires cross-pollination, creating a higher exchange of genes and leads to homogenization of genetic composition. Overall, these results indicated no genetic differentiation, meaning that all individuals remain taxonomically under the same species.

ISSN 1511-3701

e-ISSN 2231-8542

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