e-ISSN 2231-8542
ISSN 1511-3701

Home / Regular Issue / JTAS Vol. 19 (2&3) Dec. 1996 / JTAS-0119-1996


In Vitro Responses of Dracaena fragrans cv. Massangeana to Growth Regulators

Maheran A. Aziz, H.L. Ooi and A.A. Rashid

Pertanika Journal of Tropical Agricultural Science, Volume 19, Issue 2&3, December 1996

Keywords: Dracaena fragrans, Murashige and Skoog (MS) medium, BAP, NAA, 2, 4-D, shoot formation, callus, rooting

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In vitro studies on Dracaena fragrans cv. Massangeana revealed that young stem segments were capable ofProliferating shoots on agar-solidified Murashige and Skoog (MS) basal medium containing different combinations and concentrations of BAP and NAA. Highest percentage of explants forming shoots was obtained on medium supplemented with 3.0 mg 11 BAP and 0.1 mg 11 NAA. The highest number of shoots per explants occurred on medium containing 2.0 mg/l BAP only. Highest percentage of callus formation and highest mean fresh weight of callus from young stem segments were achieved on M S medium supplemented with 1.0 mg 112,4-D. Adventitious rooting occurred after transferring excised shoots onto a hormone-free M S medium. Rooting was 100 % for shoots derived from media with 0-2.0 mg/l BAP and a relatively low concentration of NAA (0.1 mg/l).

ISSN 1511-3701

e-ISSN 2231-8542

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