In vitro studies on Dracaena fragrans cv. Massangeana revealed that young stem segments were capable ofProliferating shoots on agar-solidified Murashige and Skoog (MS) basal medium containing different combinations and concentrations of BAP and NAA. Highest percentage of explants forming shoots was obtained on medium supplemented with 3.0 mg 11 BAP and 0.1 mg 11 NAA. The highest number of shoots per explants occurred on medium containing 2.0 mg/l BAP only. Highest percentage of callus formation and highest mean fresh weight of callus from young stem segments were achieved on M S medium supplemented with 1.0 mg 112,4-D. Adventitious rooting occurred after transferring excised shoots onto a hormone-free M S medium. Rooting was 100 % for shoots derived from media with 0-2.0 mg/l BAP and a relatively low concentration of NAA (0.1 mg/l).