e-ISSN 2231-8542
ISSN 1511-3701

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Gender Identification of Domesticated Chicken Using a PCR-based Method

Richard Teh Swee Aun and Jayaraj Vijaya Kumaran

Pertanika Journal of Tropical Agricultural Science, Volume 33, Issue 2, August 2010

Keywords: Monomorphism, PCR based gender determination protocol, CHD-W and CHD-Z

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The monomorphism of newly hatched chicks poses a problem for farmers who need to separate them. Currently, the most widely used technique is the cloacal sexing method which has a low accuracy rate. Thus, a PCR based gender determination protocol was established to obtain a higher accuracy rate than conventional sexing methods. Feathers of a day up to a week old chicks were collected for the molecular analysis using the PCR targeting the CHD-W and CHD-Z genes in the chicken's chromosome Z and chromosome W, respectively. The results of this study showed that the PCR based gender determination protocol was a sensitive and accurate method for determining the gender of monomorphic chicks as compared to the conventional sexing method.

ISSN 1511-3701

e-ISSN 2231-8542

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