PERTANIKA JOURNAL OF TROPICAL AGRICULTURAL SCIENCE

 

e-ISSN 2231-8542
ISSN 1511-3701

Home / Regular Issue / JTAS Vol. 26 (1) Apr. 2003 / JTAS-0261-2003

 

A Protocol for Efficient Plantlet Regeneration from Leaf Derived Callus of Lablab Bean (Lablab purpureus var. lignosus (L) prain)

Muthu Thiruvengadam and Narayanasamypillai Jayabalan

Pertanika Journal of Tropical Agricultural Science, Volume 26, Issue 1, April 2003

Keywords: Lablab purpureus, multiple shoot induction, organogenesis, tissue culture

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An efficient shoot bud differentiation and multiple shoot induction from leaf derived callus of Lablab bean (Lablab purpureus var. lignosus) have been obtained. Callus induction and shoot multiplication at various frequencies were observed using different concentrations and combinations of auxins (1AA, 2, 4-D and NAA) and cytokinin (BAP). The highest frequency of callus induction was observed on MS medium containing 2, 4-D (3.0 mg/1) and BAP (0.5 mg/1). The green compact nodular calli occurred on NAA (3.0 mg/1) and BAP (0,5 mg/1). Highest percentage of shoot bud formation and multiplication was obtained from a combination of BAP (2.0 mg/1) and NAA (0.5 mg/1). The regenerated shoots were transferred to MS medium containing IBA (1.5 mg/1) for the induction of roots. Rooted plants were transferred to plastic cups and subsequently these were successfully transferred to fields.

ISSN 1511-3701

e-ISSN 2231-8542

Article ID

JTAS-0261-2003

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