e-ISSN 2231-8542
ISSN 1511-3701

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Genetic Identification and Micropropagation of Distributed Persimmons (Diospyros kaki) in Indonesia

Novita Chrisna Wardani, Endang Yuniastuti and Sulandjari

Pertanika Journal of Tropical Agricultural Science, Volume 42, Issue 4, November 2019

Keywords: Conservation, Diospyros kaki, genetic identification, micropropagation

Published on: 13 Nov 2019

While existing persimmon productivity in Indonesia has been gradually decreasing because of improper cultural practices and environmental conditions, species conservation has become increasingly crucial given its benefit to human health. Genetic identification of the persimmon (Diospyros kaki), the type found in Indonesia, would provide valuable genetic information about this persimmon cultivar. Nine selected random amplified polymorphic DNA (RAPD) primers successfully yielded 32 polymorphic bands and 20 monomorphic bands. Amplified bands identified 20 persimmon samples that were derived from one genotype, given their similarity coefficient value of more than 0.76. The genotype was cultured under a controlled environment in order to produce clonal and healthy seedlings over a year. Although persimmon micropropagation had been successfully established at the initiation stage, but the explants growth of each treatment did not significantly differ. However, the highest mean of leaf number was achieved individually on treatment of Woody Plant Medium (WPM) and plant growth regulator (IBA 2 ppm + BAP 2 ppm); this is significant given that the number of formed leaves determines the persimmon multiplication rate. The regenerated shoot must be subcultured on modified culture medium for further persimmon micropropagation stages.

ISSN 1511-3701

e-ISSN 2231-8542

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