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Efficiency of Bioconversion of Coffee Pulp using Hermetia illucens (Diptera: Stratiomyidae) Larvae

Karen Ospina-Granobles and Nancy Carrejo-Gironza

Pertanika Journal of Tropical Agricultural Science, Volume 44, Issue 1, February 2021


Keywords: Bioconversion, coffee pulp, Hermetia illucens

Published on: 24 Febuary 2021

In this study, we evaluate the capacity of Hermetia illucens (L.) to degrade coffee pulp, which is a highly contaminating residue and a by-product of processing coffee beans. The larvae were fed with dietary dosages of 60, 100, 160, and 200 mg/larva/day of Coffea arabica (L.) Castillo variety (0.5% caffeine), until they reached pupae stage. With a dietary supply of 100 mg/larva/day, the highest reduction percentage (%R) on a wet basis (62.88%) and efficiency of conversion of ingested food (ECI) (7.89%) were achieved, while with a dietary supply of 160 mg/larva/day the highest weight reduction index (WRI) in a wet basis was achieved (0.85%). Larvae fed 200 mg/larva/day had the highest weight (115.9 mg) and shortest average development time (38.65 days). In this study we were able to define which dietary supplies of 160 and 200 mg/larva/day, allowing for efficient bioconversion of coffee pulp.

  • Alattar, M. A. (2012). Biological treatment of leachates of microaerobic fermentation [Master’s thesis, Portland State University]. Pdxscholar.

  • Arango, G., Vergara, R., & Mejía, H. (2004). Análisis composicional, microbiológico y digestibilidad de la proteína de la harina de larvas de Hermetia illucens L. (Diptera: Stratiomyidae) en Angelópolis-Antioquia, Colombia [Compositional, microbiological and digestibility analysis of the protein of the larvae meal of Hermetia illucens L. (Diptera: Stratiomyidae) in Angelopolis-Antioquia, Colombia]. Revista Facultad Nacional de Agronomía, 57(2), 2491-2499.

  • Banks, I., Gibson, W., & Cameron, M. (2014). Growth rates of black soldier fly larvae fed on fresh human faeces and their implication for improving sanitation. Tropical Medicine International Health, 19(1), 14-22.

  • Blandón, C., Rodríguez, V., & Dávila, M. (1999). Caracterización microbiológica y físico-química de los subproductos del beneficio del café en proceso de compostaje [Microbiological and physical-chemical characterization of coffee processing by-products in the composting process]. Cenicafé, 49, 169-185.

  • Bondari, K., & Sheppard, C. (1987). Soldier fly, Hermetia illucens L., larvae as feed for channel catfish, Ictalurus punctatus (Rafinesque), and blue tilapia, Oreochromis aureus (Steindachner). Aquaculture Research, 18(3), 209-220.

  • Booram, C. V. Jr., Newton, G. L., Hale, O. M., & Barker, R. W. (1977). Manure residue as a substrate for protein production via Hermetia illucens larvae.

  • Canary, G. (2009). Diseño y gestión de un proceso para reciclar desechos orgánicos con la larva Hermetia illucens para producir harina de larva [Design and management of a process to recycle organic waste with the larva Hermetia illucens to produce larva flour].

  • Diener, S., Studt, N. M., Roa, F., Zurbügg, C., & Tockner, K. (2011). Biological treatment of municipal organic waste using black soldier fly larvae. Waste Biomass Valorization, 2(4), 357-363.

  • Diener, S., Zurbrügg, C., & Tockner, K. (2009). Conversion of organic material by black soldier fly larvae: Establishing optimal feeding rates. Waste Management and Research, 27(6), 603-610.

  • Elías, L. (1978). Composición química de la pulpa de café y otros subproductos [Chemical composition of coffee pulp and other by-products]. In J. E. Braham (Ed.), Pulpa de café: Composición, tecnología y utilización [Coffee pulp: Composition, technology and use] (pp. 19-29). Institute of Nutrition of Central America and Panama.

  • Furman, D., Young, R., & Catts, P. (1959). Hermetia illucens as a factor in the natural control of Musca domestica Linnaeus. Journal of Economic Entomology, 52(5), 917-921. https://doi: 10.1177/0734242X09103838

  • Gamboa, E., & Jasbón, N. (2008). Degradación de residuos de almidón y cárnicos, mediante la producción de la larva soldado negra (Hermetia illucens L.) [Degradation of starch and meat residues by production of the black soldier larva (Hermetia illucens L.)].

  • Hale, O. M. (1973). Dried Hermetia illucens larvae (Diptera: Stratiomyidae) as a feed additive for poultry. Journal of the Georgia Entomological Society, 8, 16-20.

  • Jaramillo, G., & Zapata, L. (2008). Aprovechamiento de los residuos sólidos orgánicos en Colombia [Use of organic solid waste in Colombia].

  • Kim, W., Bae, S., Park, K., Lee, S., Choi, Y., Han, S., & Koh, Y. (2011). Biochemical characterization of digestive enzymes in the black soldier fly, Hermetia illucens (Diptera: Stratiomyidae). Journal of Asia-Pacific Entomology, 14(1), 11-14.

  • Lardé, G. (1990). Recycling of coffee pulp by Hermetia illucens (Diptera: Stratiomyidae) larvae. Biological Wastes, 33(4), 307-310. https://doi: 10.1016/0269-7483(90)90134-E

  • Lord, W. D., Goff, M. L., & Adkins, T. R. (1994). The black soldier fly Hermetia illucens (Diptera: Stratiomyidae) as a potential measure of human post-mortem interval: Observations and case histories. Journal of Forensic Sciences, 39(1), 215-22. https://doi: 10.1111/j.1556-4029.2008.00659.x

  • Manurung, R., Supriatna, A., Esyanthi, R., & Putra, R. E. (2016). Bioconversion of rice straw waste by black soldier fly larvae (Hermetia illucens L.): Optimal feed rate for biomass production. Journal of Entomology and Zoology Studies, 4(4), 1036-1041.

  • Meneguz, M., Gasco, L., & Tomberlin, J. K. (2018). Impact of pH and feeding system on black soldier fly (Hermetia illucens, L; Diptera: Stratiomyidae) larval development. PLoS One, 13(8), e0202591.

  • Molina, M., Lechuga, R., & Bressan, R. (1990). Valor nutritivo de la pulpa de café sometida a fermentación sólida usando Aspergillus niger en pollos y cerdos [Nutritional value of coffee pulp subjected to solid fermentation using Aspergillus niger in chickens and pigs]. Agronomía Mesoamericana, 1, 79-82.

  • Myers, H., Tomberlin, J., Lambert, B., & Kattes, D. (2008). Development of black soldier fly (Diptera: Stratiomyidae) larvae fed dairy manure. Environmental Entomology, 37(1), 11-15.

  • Newton, G. L., Sheppard, D. C., Watson, D. W., Burtle, G. J., Dove, C. R., Tomberlin, J. K., & Thelen, E. (2005a). The black soldier fly, Hermetia illucens, as a manure management/resource recovery tool.

  • Newton, G. L., Sheppard, D. C., Watson, D. W., Burtle, G., & Dove, R. (2005b). Using the black soldier fly, Hermetia illucens, as a value-added tool for the management of swine manure.

  • Nguyen, T., Tomberlin, J., & Vanlaerhoven, S. (2013). Influence of resources on Hermetia illucens (Diptera: Stratiomyidae) larval development. Journal of Medical Entomology, 50(4), 898-906.

  • Nijhout, H. F., & Williams, C. M. (1974). Control of molting and metamorphosis in tobacco hornworm, Manduca sexta (L): Growth of last-instar larva and decision to pupate. Journal of Experimental Biology, 61(2), 481-491.

  • Noriega, A., Silva, R., & García de Salcedo, M. (2008). Revisión: Utilización de la pulpa de café en alimentación animal [Review: Use of coffee pulp in animal feed]. Zootecnia Tropical, 26(4), 411-419.

  • Oonincx, D., Van Huis, A., & Van Loon, J. (2015a). Nutrient utilization by black soldier flies fed with chicken, pig, or cow manure. Journal of Insects as Food and Feed, 1(2), 131-139.

  • Pandey, A. K., Mishra, B. K., Arora, A., Singh, S., & Lata-Ray, R. C. (2011). Bioaugmentation and biovalourization of agro-food and beverage industry effluents. In A. Singh, N. Parmar, & R. Kuhad (Eds.) Bioaugmentation, biostimulation and biocontrol (pp. 85-106). Springer.

  • Parra, S., Carrejo, N., & Gómez, C. (2015). Effects of larval density and feeding rates on the bioconversion of vegetable waste using black soldier fly larvae Hermetia illucens (L.), (Diptera: Stratiomyidae). Waste Biomass Valorization, 6(6), 1059-1065.

  • Pujol-Luz, J. R., Francez, P. A. C., Ururahy-Rodrigues, U., & Constantino, R. (2008). The black soldier by, Hermetia illucens (Diptera, Stratiomyidae), used to estimate the postmortem interval in a case in Amapá State, Brazil. Journal of Forensic Sciences, 53(2), 476-478.

  • Rodríguez. V., & Zambran, D. (2010). Los subproductos del café: Fuente de energía renovable [Coffee by-products: A source of renewable energy]. Cenicafé Press.

  • Rubio, J. (1973). Composición química y digestibilidad in vitro de la pulpa de café [Chemical composition and in vitro digestibility of coffee pulp] [Master’s thesis, Universidad Nacional]. Universidad Nacional Research Repository.

  • Scriber, J., & Slansky, F. (1981). The nutricional ecology of immature insects. Annual Review of Entomology, 26(1), 183-211.

  • Sheppard, D. C., Newton, G. L., Thompson, S. A., & Savage, S. (1994). A value-added manure management-system using the black soldier fly. Bioresource Technology, 50(3), 275-279.

  • Sheppard, D. C., Tomberlin, J. K., Joyce, J. A., Kiser, B. C., & Sumner, S. M. (2002). Rearing methods for the black soldier fly (Diptera: Stratiomyidae). Journal of Medical Entomology, 39(4), 695-698.

  • Supriyatna, A., Manurung, R., Rachmi-Esyanti, R., & Putra, R. E. (2016). Growth of black soldier larvae fed on cassava peel wastes, an agriculture waste. Journal of Entomology and Zoology Studies, 4(6), 161-165.

  • Tomberlin, J., Shepard, D., & Joyce, J. (2002). Selected life-history traits of black soldier flies (Diptera: Stratiomyidae) reared on three artificial diets. Annals of the Entomological Society of America, 95(3), 379-386.[0379:SLHTOB]2.0.CO;2

  • Ulloa, J. A. (2007). Frutas auto estabilizadas en el envase por la tecnología de obstáculos [Fruits self-stabilized in the package by the obstacle technology]. Universidad Autónoma de Nayarit Press.

  • Waldbauer, G. P. (1968). The consumption and utilization of food by insects. In Advances in Insect Physiology, 5, 229-288.

  • Warburton, K., & Hallman, V. (2002). Processing of organic materials by the soldier fly, Hermetia illucens.

  • Zheng, L., Cai, H., Garza, E., Yu, Z., & Zhou, S. (2011). From organic waste to biodiesel: Black soldier fly, Hermetia illucens, makes if feasible. Fuel, 90(4), 1545-1548.

  • Zheng, L., Zhang, J., & Yu, Z. (2012). Double the biodiesel yield: Rearing black soldier fly larvae, Hermetia illucens, on solid residual fraction of restaurant waste after grease extraction for biodiesel production. Renewable Energy, 41, 75-79.

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