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Home / Regular Issue / JTAS Vol. 46 (2) May. 2023 / JTAS-2549-2022


Pathogenicity of Aeromonas hydrophila in High-value Native Pangasius Catfish, Pangasius nasutus (Bleeker)

Bashir Sani, Md. Sabri Yusoff, Ina Salwany Md Yasin, Murni Marlina Abd Karim, Mohd Fuad Matori, Mohammad Noor Azmai Amal, Tilusha Manchanayake, Amir-Danial Zahaludin and Aslah Mohamad

Pertanika Journal of Tropical Agricultural Science, Volume 46, Issue 2, May 2023


Keywords: Aeromonas hydrophila, LD50, pangasius catfish, Pangasius nasutus, pathogenicity

Published on: 16 May 2023

Pangasius catfish, Pangasius nasutus, is a promising candidate for aquaculture due to its high market value. However, the presence of pathogenic bacteria in Aeromonas hydrophila is a major concern in P. nasutus farming in this country. This study determines the pathogenicity of A. hydrophila in P. nasutus. A total of 80 P. nasutus juveniles were intraperitoneally injected with 0, 103, 105, and 107 CFU mL-1 of A. hydrophila and monitored until 240 hr. The infected moribund fish’s kidneys, livers, and spleens were collected for histopathological analysis. The LD50-240hr value was found at 0.8 × 104 CFU/ml of A. hydrophila. The percentage of mortality in 0, 103, 105, and 107 CFU/ml infected groups were found to be at 0, 40, 60, and 90%, respectively. The infected fish showed congestion at the base of the fin, ascites, enlarged gall bladder, and swollen spleen. It is the earliest report on A. hydrophila’s pathogenicity in high-value native fish, P. nasutus.

  • Abdelhamed, H., Ibrahim, I., Nho, S. W., Banes, M. M., Wills, R. W., Karsi, A., & Lawrence, M. L. (2017). Evaluation of three recombinant outer membrane proteins, OmpA1, Tdr, and TbpA, as potential vaccine antigens against virulent Aeromonas hydrophila infection in channel catfish (Ictalurus punctatus). Fish and Shellfish Immunology, 66, 480–486.

  • Anjur, N., Sabran, S. F., Daud, H. M., & Othman, N. Z. (2021). An update on the ornamental fish industry in Malaysia: Aeromonas hydrophila-associated disease and its treatment control. Veterinary World, 14(5), 1143–1152.

  • Azzam-Sayuti, M., Ina-Salwany, M. Y., Zamri-Saad, M., Annas, S., Yusof, M. T., Monir, M. S., Mohamad, A., Muhamad-Sofie, M. H. N., Lee, J. Y., Chin, Y. K., Amir-Danial, Z., Asyiqin, A., Lukman, B., Liles, M. R., & Amal, M. N. A. (2021). Comparative pathogenicity of Aeromonas spp. in cultured red hybrid tilapia (Oreochromis niloticus × O. mossambicus). Biology, 10(11), 1192.

  • Azzam-Sayuti, M., Ina-Salwany, M. Y., Zamri-saad, M., Yusof, M. T., Annas, S., Najihah, M. Y., Liles, M. R., Monir, M. S., Zaidi, Z., Amal, M. N. A., Ina-salwany, Y., Zamri-saad, M., Monir, S., Zaidi, Z., Noor, M., & Amal, A. (2021). The prevalence, putative virulence genes and antibiotic resistance profiles of Aeromonas spp. isolated from cultured freshwater fishes in peninsular Malaysia. Aquaculture, 540, 736719.

  • Bøgwald, J., & Dalmo, R. A. (2019). Review on immersion vaccines for fish: An update 2019. Microorganisms, 7(12), 627.

  • Chen, N., Jiang, J., Gao, X., Li, X., Zhang, Y., Liu, X., Yang, H., Bing, X., & Zhang, X. (2018). Histopathological analysis and the immune related gene expression profiles of mandarin fish (Siniperca chuatsi) infected with Aeromonas hydrophila. Fish and Shellfish Immunology, 83, 410–415.

  • de Oliveira, S. R., de Souza, R. T. Y. B., Brasil, E. M., de Andrade, J. I. A., da Silva Santiago Nunes, É., Ono, E. A., & Affonso, E. G. (2011). LD50 of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus. Acta Amazonica, 41(2), 321–326.

  • Dias, M. K. R., Sampaio, L. S., Proietti, A. A., Yoshioka, E. T. O., Rodrigues, D. P., Rodriguez, A. F. R., Ribeiro, R. A., Faria, F. S. E. D. V., Ozório, R. O. A., & Tavares-Dias, M. (2016). Lethal dose and clinical signs of Aeromonas hydrophila in Arapaima gigas (Arapaimidae), the giant fish from Amazon. Veterinary Microbiology, 188, 12–15.

  • Doan, H. V., Suksri, A., & Pensée, L. (2013). The LD50 of Asian catfish (Pangasius bocourti, Sauvage 1870) challenge to pathogen Aeromonas hydrophila FW52 strain. Pensee Journal, 75(10), 287–293.

  • Dong, H. T., Techatanakitarnan, C., Jindakittikul, P., Thaiprayoon, A., Taengphu, S., Charoensapsri, W., Khunrae, P., Rattanarojpong, T., & Senapin, S. (2017). Aeromonas jandaei and Aeromonas veronii caused disease and mortality in Nile tilapia, Oreochromis niloticus (L.). Journal of Fish Diseases, 40(10), 1395–1403.

  • Hashim, R. B., Jamil, E. F., Zulkipli, F. H., & Daud, J. M. (2015). Fatty acid compositions of silver catfish, Pangasius sp. farmed in several rivers of Pahang, Malaysia. Journal of Oleo Science, 64(2), 205–209.

  • Hayati, R. L., & Prihanto, A. A. (2020). Effects of polysaccharides-crude extract from Candida sp. OCL1 on hematological parameters of Aeromonas hydrophila-infected catfish (Pangasius pangasius). In IOP Conference Series: Earth and Environmental Science (Vol. 493, No. 1, p. 012016). IOP Publishing.

  • Jaapar, M. Z., Yusof, M. F., Yusof, H. M., Ramli, S. F., Mohamad, S. N., & Jamari, Z. (2021). Effect of different salinity concentrations on hatching rate and larval development of Patin buah, Pangasius nasutus (Bleeker, 1863). Journal of Applied Aquaculture, 34(3), 693-701.

  • Jamaludin, F. I., & Ting, S. Y. (2021). Penilaian potensi bahan mentah tempatan dalam formulasi makanan ikan patin [Assessment of the potential of local raw materials in the formulation of catfish food]. Buletin Teknologi MARDI, 28(2), 145–152.

  • Jiang, X., Zhang, C., Zhao, Y., Kong, X., Pei, C., Li, L., Nie, G., & Li, X. (2016). Immune effects of the vaccine of live attenuated Aeromonas hydrophila screened by rifampicin on common carp (Cyprinus carpio L.). Vaccine, 34(27), 3087–3092.

  • Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution, 35(6), 1547-1549.

  • Laith, A. R., & Najiah, M. (2014). Aeromonas hydrophila: Antimicrobial susceptibility and histopathology of isolates from diseased catfish, Clarias gariepinus (Burchell). Journal of Aquaculture Research and Development, 5(2), 1000215.

  • Le, T. S., Nguyen, T. H., Vo, H. P., Doan, V. C., Nguyen, H. L., Tran, M. T., Tran, T. T., Southgate, P. C., & İpek Kurtböke, D. (2018). Protective effects of bacteriophages against Aeromonas hydrophila species causing Motile Aeromonas Septicemia (MAS) in striped catfish. Antibiotics, 7(1), 16.

  • Mansor, N. N., Khan Chowdhury, A. J., Ridzuan, M. S., Mohd Khalid, H. N., & Mahmood, S. (2020). Epidemiology of emerging diseases and disorder in cage cultured Pangasius spp. in Pahang, Malaysia. International Islamic University Malaysia Repository.

  • Matusin, S. B. (2015). Molecular characterization of Aeromonas hydrophila and development of recombinant cells vaccine expressing outer membrane proteins against its in African catfish (Clarias gariepinus) [Unpublished Master’s thesis]. Universiti Putra Malaysia.

  • Mazumder, A., Choudhury, H., Dey, A., & Sarma, D. (2021). Isolation and characterization of two virulent Aeromonads associated with haemorrhagic septicaemia and tail-rot disease in farmed climbing perch Anabas testudineus. Scientific Reports, 11, 5826.

  • Rafi, A. I. A., Hanan, M. Y., Othman, A. baihaqi, Jaapar, M. Z., & Baharuddin, H. (2022). Potential usage of Sirehmax™, a piper betle extract, for controlling fish pathogen prevalence in domesticated shark fingerlings, Pangasius nasutus (Bleeker, 1863). Fishmail, 31, 32–36.

  • Rahman, A. A. N., Mansour, D. A., Abd El-Rahman, G. I., Elseddawy, N. M., Zaglool, A. W., Khamis, T., Mahmoud, S. F., & Mahboub, H. H. (2022). Imidacloprid toxicity in Clarias gariepinus: Protective role of dietary Hyphaene thebaica against biochemical and histopathological disruption, oxidative stress, immune genes expressions, and Aeromonas sobria infection. Aquaculture, 555, 738170.

  • Reed, L. J., & Muench, H. (1938). A simple method of estimating fifty per cent endpoints. American Journal of Epidemiology, 27(3), 493–497.

  • Saharia, P., Pokhrel, H., Kalita, B., Hussain, I. A., & Islam, S. (2018). Histopathological changes in Indian Major Carp, Labeo rohita (Hamilton), experimentally infected with Aeromonas hydrophila associated with hemorrhagic septicemia of Central Brahmaputra valley of Assam, India. Journal of Entomology and Zoology Studies, 6(5), 6–11.

  • Samayanpaulraj, V., Velu, V., & Uthandakalaipandiyan, R. (2019). Microbial pathogenesis determination of lethal dose of Aeromonas hydrophila Ah17 strain in snake head fish Channa striata. Microbial Pathogenesis, 127, 7–11.

  • Sarker, J., & Faruk, M. (2016). Experimental infection of Aeromonas hydrophila in pangasius. Progressive Agriculture, 27(3), 392–399.

  • Sirimanapong, W., Thompson, K. D., Kledmanee, K., Thaijongrak, P., Collet, B., Ooi, E. L., & Adams, A. (2014). Optimisation and standardisation of functional immune assays for striped catfish (Pangasianodon hypophthalmus) to compare their immune response to live and heat killed Aeromonas hydrophila as models of infection and vaccination. Fish and Shellfish Immunology, 40(2), 374–383.

  • Tahapari, E., Darmawan, J., & Abdurachman, M. L. (2020). Nuclear DNA content variation within four species of Asian catfish of family Pangasidae and their two interspecific hybrids by flow cytometry. Jurnal Akuakultur Indonesia, 19(2), 142–152.

  • Tartor, Y. H., EL-Naenaeey, E.-S. S. Y., Abdallah, H. M., Samir, M., Yassen, M. M., & Abdelwahab, A. M. (2021). Virulotyping and genetic diversity of Aeromonas hydrophila isolated from Nile tilapia (Oreochromis niloticus) in aquaculture farms in Egypt. Aquaculture, 541, 736781.

  • Wohlsen, T., Bates, J., Vesey, G., Robinson, W. A., & Katouli, M. (2006). Evaluation of the methods for enumerating coliform bacteria from water samples using precise reference standards. Letters in Applied Microbiology, 42(4), 350–356.

  • Yusof, F., & Nakajima, M. (2019). Morphological tool to elucidate two closely related pangasius catfish. International Journal of Allied Health Sciences, 3(2), 967–975.

  • Zhang, D., Xu, D.-H., & Beck, B. (2019). Analysis of agglutinants elicited by antiserum of channel catfish immunized with extracellular proteins of virulent Aeromonas hydrophila. Fish and Shellfish Immunology, 86, 223–229.

  • Zhang, D., Xu, D.-H., & Shoemaker, C. (2016). Experimental induction of motile Aeromonas septicemia in channel catfish (Ictalurus punctatus) by waterborne challenge with virulent Aeromonas hydrophila. Aquaculture Reports, 3, 18–23.

  • Zulkiflee, S. Z., Yusof, M. F., Nor Azlina, A. R., & Rostam, M. A. (2020). Moisture, ash and fat composition of Pangasianodon hypophthalmus (Sauvage, 1878) and Pangasius nasutus (Bleeker, 1863). International Journal of Allied Health Sciences, 4(3), 1486–1495.

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