Reinvestigation oftheflavonoid glycosides ofthe speaVsL. japonicum indicated the presence of quercetin 3-O-rutinoside, vicenin-2, kaempferol O-glycosides and vitexin. Thus, the flavonoid data at present seem to be differentexcept for the presence of vitexin) from those of Hiraoka (1978). The differences in flavonoid patterns between L. japonicum from Japan and L. japonicum from Peninsular Malaysia suggest geographical variation. In addition to the extraction of flavonoids in L. japonicum in the present study, quercetin 3-O-rutinoside and vicenin-2 were reported for the first time in the family Athyriaceae.