e-ISSN 2231-8542
ISSN 1511-3701

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The Use of Antibody-sensitized Latex to Detect Cymbidium Mosaic Virus in Orchids

Norani Abdul-Samad and Zainab Ari

Pertanika Journal of Tropical Agricultural Science, Volume 16, Issue 2, August 1993

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Latex agglutination test (L) and a modification of the latex test, protein A-coated latex (PAL) were compared for sensitization with antibodies to Cymbidium mosaic virus (CyMV). A series of globulin concentrations was tested against purified CyMV. The minimum detectable virus concentration was similar for both L and PAL reactions at 0.104 µg/ml The detectable end points in infected Oncidium Gower Ramsey orchid sap were similar at a dilution of 1/2560. PAL was found to be more sensitive than L at a higher globulin dilution of 1/512. The PAL test showed higher sensitivity than L based on five orchid hybrids and compared favourably with electron microscopy test.

ISSN 1511-3701

e-ISSN 2231-8542

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