e-ISSN 2231-8542
ISSN 1511-3701

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Histological Observations of Adventitious Root Derived from in vitro Plantlet and Shoot Bud of Boesenbergia rotunda (Zingiberaceae)

Siti Zulaiha Ahamad Azhar, Khairunnisa Abdul Ghani and Nor Azma Yusuf

Pertanika Journal of Tropical Agricultural Science, Volume 42, Issue 2, May 2019

Keywords: Adventitious root, Boesenbergia rotunda, histological, in vitro, plantlet, shoot bud

Published on: 30 May 2019

Boesenbergia rotunda or locally known as 'Temu kunci' is renowned to possess some important bioactive compounds that are promising for pharmacological applications. It is essential to obtain structural information of the inner parts of the in vitro roots since growing these roots undertake various anatomical and morphological changes that influence their cells activities and nutrient uptake processes. Although root functions are thought to be significant for the growth of shoot, the morphological and anatomical knowledge of adventitious root for this plant are limited. This particular study aims to investigate and compare anatomical structures of in vitro adventitious root derived from in vitro plantlet with root derived from shoot bud of B. rotunda. Histological sections using resin were done to study the anatomical of adventitious root of B. rotunda. The root samples were fixed in a Glutaraldehyde-Paraformaldehyde-Caffeine (GPC) fixative, dehydrated, infiltrated, embedded, cut, stained and mounted with Surgipath mounting medium for observations under light microscope. From the histological observations, adventitious roots of B. rotunda had shown the presence of all the three main tissue systems and had the same internal structures containing epidermis, exodermis, suberized sclerenchyma cells, cortex and stele. Both adventitious roots derived from in vitro plantlet and shoot bud showed normal growth morphologically, had anatomically same normal cell structures and arrangements.

ISSN 1511-3701

e-ISSN 2231-8542

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