e-ISSN 2231-8542
ISSN 1511-3701

Home / Regular Issue / JTAS Vol. 42 (2) May. 2019 / JTAS-1647-2018


Effect of Glucose and Ascorbic Acid on Total Phenolic Content Estimation of Green Tea and Commercial Fruit Juices by Using Folin Ciocalteu and Fast Blue BB Assays

Ahmad Syazrin Roslan, Yoshito Ando, Azrina Azlan and Amin Ismail

Pertanika Journal of Tropical Agricultural Science, Volume 42, Issue 2, May 2019

Keywords: Ascorbic acid, Fast Blue BB assay, Folin Ciocalteu assay, green tea, total phenolic content

Published on: 30 May 2019

Folin Ciocalteu (FC) assay had been widely used in the estimation of total phenolics content (TPC) in foods. However, the main disadvantage of this assay is that the reagent reacts with reducing substances and measures the total reducing capacity of a sample, not just phenolic compounds. The Fast Blue BB (FBBB) assay is another assay that can be used to estimate the total phenolic content in foods instead. The aim of the study was to estimate and compare the total phenolic content of green tea and commercial fruit juices using FC and FBBB assays, and also the effects of glucose and ascorbic acid on both assays. Green tea had the highest ascorbic acid content and TPC value among the samples. TPC estimated using FC assay were significantly higher than FBBB assay in all the samples. FC and FBBB assays were not affected by glucose at different concentrations. However, FC assay was significantly affected by the presence of ascorbic acid compared to FBBB assay. In conclusion, FC assay overestimated the TPC value in the sample extracts and was significantly affected by the presence of ascorbic acid. The FBBB assay can be considered as an alternative for estimation of total phenolic content in ascorbic acid-rich foods.

ISSN 1511-3701

e-ISSN 2231-8542

Article ID


Download Full Article PDF

Share this article

Recent Articles