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Use of Amplified Fragment Length Polymorphism and Sequence Characterized Amplified Region Marker for Identifying the Sex of the Oxyeleotris marmorata

Douglas Law, Voon Chun Ping, Tan Chin Yee, Herryawan Ryadi Eziwar Dyari, Maizan Haiji Mohamed, Shazrul Fazry and Nik Marzuki Sidik

Pertanika Journal of Tropical Agricultural Science, Volume 44, Issue 1, February 2021


Keywords: AFLP, Oxyeleotris marmorata, sex determination, sex marker

Published on: 24 Febuary 2021

There is a huge demand for the Oxyeleotris marmorata, especially in Asian markets. However, farmers are unable to provide a constant supply of this fish to meet the demand, which is estimated to be around 100 metric tonnes per annum. One of the reasons that are hindering the supply is the low success rate of O. marmorata breeding programs. These breeding programs rely on many factors for their success, one of which is the use of genuine male and female adults, although determining these could be a daunting task. This research was carried out in an attempt to determine a sex marker for the O. marmorata using the amplified fragment length polymorphism (AFLP) method. Of the 30×30 AFLP primer mixtures screened, the E-TAA and M-CTT primer pair had an amplified ~600 bp marker that was specific to the female. This ~600 bp AFLP marker was later used to design a 464 bp sequence characterized amplified region (SCAR) marker. Thus, it has been suggested that the SCAR marker obtained has the potential to be used for the sexual identification of the O. marmorata at the juvenile stage, thereby enabling them to be used in breeding programs.

  • Al-Ameri, A. A., Al-Qurainy, F., Gaafar, A. R. Z., Khan, S., & Nadeem, M. (2016). Molecular identification of sex in Phoenix dactylifera using inter simple sequence repeat markers. BioMed Research International, 2016, 4530846.

  • Blessing, J. J., Marshall, J. C., & Balcome, S. R. (2010). Humane killing of fishes for scientific research: A comparison of two methods. Journal of Fish Biology, 76(10), 2571-2577. 3.x

  • Cheng, D., Mei, J., Wang, D., & Gui, J. F. (2013). Genetic differentiation and efficient sex-specific marker development of a pair of Y- and X-linked markers in yellow catfish. International Journal of Biological Science, 9(10), 1043–1049.

  • Felip, A., Martinez-Rodriguez, G., Piferrer, F., Carrillo, M., & Zanuy, S. (2000). AFLP analysis confirms exclusive maternal genomic contribution of meiogynogenetic sea bass (Dicentrarchus labrax L.). Marine Biotechnology, 2(3), 301–3066.

  • Felip, A., Young, W. P., Wheeler, P. A., & Thorgaard, G. H. (2005). An AFLP-based approach for the identification of sex-linked markers in rainbow trout (Oncorhynchus mykiss). Aquaculture, 247(1-4), 35–43.

  • Herawati, T., Yustiati, A., Nurhayati, A., & Natadia, S. S. (2016). Domestication of marble goby [Oxyeleotris marmorata (Bleeker, 1852)] indogenous fish of Citarum River, Indonesia. Aquatic Procedia, 7, 247-253.

  • Idris, H. B., Ambak, M. A., & Ikhwanuddin, M. (2012). Sex determination in Oxyeleotris marmorata (Bleeker, 1852) based on morphometric features. Advances in Natural Applied Sciences, 6(6), 763-771.

  • Kovács, B., Egedi, S., Bártfai, R., & Orbán, L. (2000). Male-specific DNA markers from African catfish (Clarias gariepinus). Genetica, 110(3), 267-276.

  • Lam, S. S., Ambak, M. A., Jusoh, A., & Law, A. T. (2014a). Growth performance and waste excretion of marble goby (Oxyeleotris Marmorata Bleeker) in relation to different culture system and diet. Aquaculture, 29(1), 41-45. j.aquaculture. 2007.11.023

  • Lam, S. S., Ma, N. L., Jusoh, A., & Ambak, M. A. (2014b). A study on the optimal tank design and feed type to the growth of marble goby (Oxyeleotris marmorata Bleeker) and reduction of waste in a recirculating aquaponic system. Desalination and Water Treatment, 52(4), 1044-1053.

  • Liu, H., Pang, M., Yu, X., Zhou, Y., Tong, J., & Fu, B. (2018). Sex-specific markers developed by next-generation sequencing confirmed an XX/XY sex determination system in bighead carp (Hypophthalmichehys nobilis) and silver carp (Hypophthalmichthys molitrix). DNA Research, 25(3), 257–264.

  • Pan, Z. J., Li, X. Y., Zhou, F. J., Qiang, X. G., & Gui, J. F. (2015). Identification of sex-specific markers reveals male heterogametic sex determination in Pseudobagrus ussuriensis. Marine Biotechnology, 17(4), 441–451.

  • Purcell, C. M., Seetharam, A. S., Snodgrass, O., Ortega-García, S., Hyde, J. R., & Severin, A. J. (2018). Insights into teleost sex determination from the Seriola dorsalis genome assembly. BMC Genomics, 19(1), 31.

  • Sambrook, J., Fritsch, E. F., & Maniatis, T. (1989). Molecular cloning: A laboratory manual (2nd ed.). Cold Spring Harbour Press.

  • Seetapan, K., Puanglarp, N., & Meunpol, O. (2012, November 22-24). Study of optimal culture conditions for juvenile marble goby (Oxyeleotris marmorata Bleeker, 1852) [Paper presentation]. Proceedings of 2nd the Annual International Conference Syiah Kuala University-Life Sciences and Engineering, Banda Aceh, Indonesia.

  • Vale, L., Dieguez, R., Sánchez, L., Martínez, P., & Viñas, A. (2014). A sex-associated sequence identified by RAPD screening in gynogenetic individuals of turbot (Scophthalmus maximus). Molecular Biology Report, 41(3), 1501–1509.

  • Vos, P., Hoger, R., & Blecker, M. (1995). AFLP: A new technique for DNA fingerprinting. Nucleic Acids Research, 23(21), 4407–4414.

  • Wang, D. W., Li, Y., & Li, Z. Q. (2011). Identification of a male-specific amplified fragment length polymorphism (AFLP) and a sequence characterized amplified region (SCAR) marker in Eucommia ulmoides Oliv. International Journal of Molecular Sciences, 12(1), 857-864.

  • Xiao, T. Q., Lu, C. Y., Li, C., Cheng, L., Cao, D. C., & Sun, X. W. (2014). An AFLP-based approach for the identification of sex-linked markers in Amur sturgeon Acipenser schrenckii Brandt, 1869. Journal of Applied Ichthyology, 30(6), 1282-1285.

  • Yong, S., Ooi, S., & Shapawi, R. (2013). The utilization of soybean meal in formulated diet for marble goby, Oxyeleotris marmoratus. Journal of Agricultural Science, 5(11), 139-149.

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