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Spore Germination of Diplazium simplicivenium Holtt. (Athyriaceae) in Peninsular Malaysia

Nurul Nadhirah, Haja Maideen, Ab Rahman Zuraida and Othman Ayu Nazreena

Pertanika Journal of Tropical Agricultural Science, Volume 47, Issue 2, May 2024


Keywords: Diplazium, fern, in vitro, micropropagation, spore

Published on: 30 May 2024

Diplazium simplicivenium Holtt. a species of fern in Peninsular Malaysia. This study reports an efficient method for D. simplicivenium spore sterilisation and the effect of plant growth regulators (PGRs) via green globular bodies (GGB). Sterilisation with 0.1% mercuric chloride effectively allowed spore germination. The result showed that the culture media supplemented with 0.5 mg/L gibberellin positively affects the weight of GGB and the number of shoots with significant differences (ANOVA, p < 0.05). The soaking technique established in this study for spore sterilisation is an efficient approach, and the optimal plant and concentration of plant hormones were identified. This procedure can be applied to other indigenous ferns and closely similar species.

  • Abrori, F. M., Saraswati., Wijarini, F., & Fatmawati. (2022). Introducing the ferns through comics: Visualisation of ethnopteridology study of Dayak Lundayeh tribe. In IOP Conference Series: Earth and Environmental Science (Vol. 1083, No. 1, p. 012013). IOP Publishing.

  • Babenko, L. M., Romanenko, K. O., Shcherbatiuk, M. M., Vasheka, O. V., Romanenko, P. O., Negretsky, V. A., & Kosakivska, I. V. (2018). Effects of exogenous phytohormones on spore germination and morphogenesis of Polystichum aculeatum (L.) Roth gametophyte in vitro culture. Cytology and Genetics, 52, 117–126.

  • Chou, H.-M., Huang, Y.-M., Wong, S.-L., Hsieh, T.-H., Hsu, S.-Y., & Chiou, W.-L. (2007). Observations of gametophytes and juvenile sporophytes of Archangiopteris somaí Hayata (Marattiaceae), an endangered fern in Taiwan. Botanical Studies, 48, 205-213.

  • Dyer, A. F. (1979). The experimental biology of ferns. Transactions of the Botanical Society of Edinburgh, 43(2), 75-90.

  • Fernández, H., & Revilla, M. A. (2003). In vitro culture of ornamental ferns. Plant Cell, Tissue and Organ Culture, 73, l-13.

  • Fernández, H., Bertrand, A. M., Feito, I., & Sanchez-Tames, R. (1997). Gametophyte culture in vitro and antheridiogen activity in Blechnum spicant. Plant Cell, Tissue and Organ Culture, 50, 71–74.

  • Golamaully, Z. M., Bhoyroo, V., Nazurally, N., & Gopal, V. (2015). In vitro cultures as an aid to conservation of indigenous ferns: Diplazium proliferum. International Journal of Plant Biology, 6(1), 6020.

  • Hollingsworth, S. N., Andres, E. A., & Greer, G. K. (2012). Pheromonal interactions among gametophytes of Osmundastrum cinnamomeum and the origins of antheridiogen systems in leptosporangiate ferns. International Journal of Plant Sciences, 173(4), 382–390.

  • Kaźmierczak, A. (1998). Studies on morphology and metabolism of prothalli during GA3-induced formation of antheridia in Anemia phyllitidis. Acta Physiologiae Plantarum, 20, 277–283.

  • Kaźmierczak, A. (2003). Induction of cell division and cell expansion at the beginning of gibberellin A3-induced precocious antheridia formation in Anemia phyllitidis gametophytes. Plant Science, 165(5), 933–939.

  • Maideen, H. M. K, Salleh, N. I. M., & Khaduwi, N. A. M. (2020). Paku pohon Cyatheaceae [Cyatheaceae tree spikes]. Penerbit Universiti Kebangsaan Malaysia.

  • Maideen, H., Farhana, N., Nadirah, N., Khaduwi, N. A., & Norhazrina, N. (2019). Ferns and lycophytes of Pulau Pangkor and its vicinity. The Malaysian Forester, 82(1), 77–86.

  • Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497.

  • Nadhirah, N., Maideen, H., Rahman, Z. A., & Othman, A. N. (2022). Optimizing in vitro surface sterilization of Cyathea latebrosa spore. Malaysian Applied Biology, 51(5), 159–163.

  • Ravi, B. X., Robert, J., & Gabriel, M. (2014). In vitro spore germination and gametophytic growth development of a critically endangered fern Pteris tripartite Sw. African Journal of Biotechnology, 13(23), 2350-2358.

  • Sharpe, J. M, Mehltreter, K., & Walker, L. R. (2010). Ecological importance of ferns. In K. Mehltreter & L. R. Walker (Eds.), Fern ecology (pp. 1-21). Cambridge University Press.

  • Taha, R. M., Haron, N. W., & Wafa, S. N. (2011). Morphological and tissue culture studies of Platycerium coronarium, a rare ornamental fern species from Malaysia. American Fern Journal, 101(4), 241–251.

  • Takeno, K., & Furuya, M. (1977). Inhibitory effect of gibberellins on archegonial differentiation in Lygodium japonicum. Physiologia Plantarum, 39(2), 135–138.

  • Wu, H., Chen, P.-T., Yuan, L.-P., & Chen, L.-Q. (2009). An efficient method for surface sterilization and sowing fern spores in vitro. American Fern Journal, 99(3), 226–230.

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