Ten enzymes and proteins: transferrin, amylase, haemoglobin, esterase D, red cell acid phosphatase, superoxide dismutase, phosphoglycolate phosphatase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and soluble glutamate oxaloacetate transaminase, from the serum and red blood cell of 88 water buffaloes, Bubalus bubalis, have been investigated by starch gel or poly aery lamide gel electrophoresis. Four of these: transferrin, amylase, haemoglobin and esterase D show electrophoretic variation at polymorphic proportions.