The learning of spectroscopic techniques for structural elucidation of unknown compounds is of profound importance to students. At present, there have been limited exercises associated with basic knowledge in the organic spectroscopy course. In this paper, through the implementation of Uncritical Inference Test (UIT) along with the teaching of organic spectroscopy course, this activity has enabled students to revise on their basic knowledge and understanding, as well as to enable the course instructor to check and rectify any weaknesses in the subsequent lessons. Three case studies based on lessons associated with Mass Spectroscopy (MS), Infrared (IR) and Nuclear Magnetic Resonance (NMR) were designed for this study. Based on the students' reflective writing, the UIT has promoted several positive learning outcomes, which includes a deeper understanding of a concept, improved thinking skills, motivated to study, fun-filled learning, peer learning and autonomous learning, which further encouraged the use of UIT in the teaching of other subjects.