In Malaysia, there is a demand and a promising market for cassava (Manihot esculanta, Crantz) leaves as a supplementary animal feed because of their nutritional value and availability throughout the year. However, cyanide accumulation has been a problem due to its toxicity in animal feed; therefore, finding the best variety with low cyanide, high protein, and phytochemical content can address this issue. The hydrocyanic (HCN) contents were analyzed for two local varieties, White and Pulut cassava, distinguished from leaf shapes and the color of the leaf petioles. Young leaves were identified from the plant’s top leaves, while matured leaves were defined from the plant’s bottom leaves. Two-way ANOVA was conducted to determine the interaction between the maturity and variety of cassava leaves for the cyanide and protein concentrations with Tukey’s multiple ranges to observe the significant difference at p < 0.05. The findings indicated significant differences in the HCN content of cassava leaves between different maturities, while other varieties significantly affected protein concentration. The maturity and variety of cassava leaves showed significant interactions with the HCN content. The young Pulut variety had the highest protein concentration and low HCN content. Thus, it is the best option as an animal feed by reducing its HCN content and maintaining its total phenolic (TPC) and total flavonoid content (TFC). The results imply that variety, as well as maturity, have significant effects on the protein and cyanide concentration of cassava leaves.

• Ajayi, M. O., & Joseph, E. (2019). Characteristics of ensiled cassava leaves and maize stover as dry season feed for ruminants. Nigerian Journal of Animal Production, 46(4), 273-281.

• Awoyinka, A. F., Abegunde, V. O., & Adewusi, S. R. A. (1995). Nutrient content of young cassava leaves and assessment of their acceptance as a green vegetable in Nigeria. Plant Foods for Human Nutrition, 47(1), 21-28. https://doi.org/10.1007/BF01088163

• Bonjoch, N. P., & Tamayo, P. R. (2001). Protein content quantification by bradford method. In Handbook of Plant Ecophysiology Techniques (pp. 283-295). Kluwer Academic Publishers. https://doi.org/10.1007/0-306-48057-3_19

• Chaiareekitwat, S., Latif, S., Mahayothee, B., Khuwijitjaru, P., Nagle, M., Amawan, S., & Müller, J. (2022). Protein composition, chlorophyll, carotenoids, and cyanide content of cassava leaves (Manihot esculenta Crantz) as influenced by cultivar, plant age, and leaf position. Food Chemistry, 372, Article 131173. https://doi.org/10.1016/j.foodchem.2021.131173

• CIAT. (1983). Morphology of the Cassava Plant. Centro Internacional de Agricultura Tropical. http://ciat-library.ciat.cgiar.org/articulos_ciat/books/Morphology_of_the_casava_plant.pdf

• da Silva Santos, B. R., Requião Silva, E. F., Minho, L. A. C., Brandão, G. C., Pinto dos Santos, A. M., Carvalho dos Santos, W. P., Lopes Silva, M. V., & Lopes dos Santos, W. N. (2020). Evaluation of the nutritional composition in effect of processing cassava leaves (Manihot esculenta) using multivariate analysis techniques. Microchemical Journal, 152, Article 104271. https://doi.org/10.1016/j.microc.2019.104271

• Dragoș, D., Petran, M., Gradinaru, T. C., & Gilca, M. (2022). Phytochemicals and inflammation: Is bitter better? Plants, 11(21), Article 2991. https://doi.org/10.3390/plants11212991

• Ekpo, U. A., & Baridia, D. F. (2020). Effect of processing on the chemical and anti-nutritional properties of cassava leaves (sweet and bitter varieties). ARC Journal of Nutrition and Growth, 6(2), 6-12. https://doi.org/10.20431/2455-2550.0602002

• Eleazu, C. O., & Eleazu, K. C. (2012). Determination of the proximate composition, total carotenoid, reducing sugars and residual cyanide levels of flours of 6 new yellow and white cassava (Manihot esculenta Crantz) Varieties. American Journal of Food Technology, 7(10), 642-649. https://doi.org/10.3923/ajft.2012.642.649

• Fritz, R. S., Hochwender, C. G., Lewkiewicz, D. A., Bothwell, S., & Orians, C. M. (2001). Seedling herbivory by slugs in a willow hybrid system: Developmental changes in damage, chemical defense, and plant performance. Oecologia, 129(1), 87-97. https://doi.org/10.1007/s004420100703

• Gensa, U. (2019). Review on cyanide poisoning in ruminants. Synthesis, 9(6), 1-12. https://doi.org/10.7176/JBAH

• Ghasemzadeh, A., Nasiri, A., Jaafar, H. Z. E., Baghdadi, A., & Ahmad, I. (2014). Changes in phytochemical synthesis, chalcone synthase activity and pharmaceutical qualities of sabah snake grass (Clinacanthus nutans L.) in relation to plant age. Molecules, 19(11), 17632-17648. https://doi.org/10.3390/molecules191117632

• Hosni, S., Gani, S. S. A., Orsat, V., Hassan, M., & Abdullah, S. (2023). Ultrasound-assisted extraction of antioxidants from Melastoma malabathricum Linn.: Modeling and optimization using Box–Behnken design. Molecules, 28(2), Article 487. https://doi.org/10.3390/molecules28020487

• Idris, S. B., Shamsudin, R., Nor, M. Z. M., Mokhtar, M. N., & Ghani, S. S. A. (2021). Proximate composition of different parts of white cassava (Manihot esculenta Crantz) plant as a ruminant feed. Advances in Agricultural and Food Research Journal, 2(1), 1-9. https://doi.org/10.36877/aafrj.a0000181

• Jamil, S. S., & Bujang, A. (2016). Nutrient and antinutrient composition of different variety of cassava (Manihot esculenta Crantz) leaves. Jurnal Teknologi, 78(6-6), 59-63. https://doi.org/10.11113/jt.v78.9024

• Lansche, J., Awiszus, S., Latif, S., & Müller, J. (2020). Potential of biogas production from processing residues to reduce environmental impacts from cassava starch and crisp production - A case study from Malaysia. Applied Sciences, 10(8), 2975. https://doi.org/10.3390/APP10082975

• Latif, S., & Müller, J. (2015). Potential of cassava leaves in human nutrition: A review. Trends in Food Science & Technology, 44(2), 147-158. https://doi.org/10.1016/j.tifs.2015.04.006

• Makkar, H. P., Siddhuraju, P., & Becker, K. (2007). Plant secondary metabolites (Vol. 393, pp. 1-122). Humana Press.

• Müller-Schwarze, D. (2009). Hands-on chemical ecology: Simple field and laboratory exercises. Springer Science & Business Media.

• Nambisan, B., & Sundaresan, S. (1994). Distribution of linamarin and its metabolising enzymes in cassava tissues. Journal of the Science of Food and Agriculture, 66(4), 503-507.

• Nwokoro, O., Ogbonna, J. C., & Okpala, G. N. (2010). Simple picrate method for the determination of cyanide in cassava flour. Bio-Research, 7(2), 502-504. https://doi.org/10.4314/br.v7i2.56582

• Nur, F. O., Siti, A. H., & Umi, K. Y. (2013). Comparative evaluation of organic and inorganic fertilizers on total phenolic, total flavonoid, antioxidant activity and cyanogenic glycosides in cassava (Manihot esculenta). African Journal of Biotechnology, 12(18), 2414-2421.

• Ojiambo, O. C., Nawiri, M. P., & Masika, E. (2017). Reduction of cyanide levels in sweet cassava leaves grown in Busia County, Kenya based on different processing methods. Food Research, 1(3), 97-102.

• Pendak, Y. (2011). Baloi: Sweet-type Tapioca Variety Suitable for Planting on Acid Sulphate Soil. Jabatan Pertanian Sarawak. http://myagric.upm.edu.my/id/eprint/12062

• Ravindran, V. (1992). Preparation of cassava leaf products and their use as animal feeds. FAO Animal Production and Health Paper, 95, 111-125.

• Roslim, D. I., Herman, Sofyanti, N., Chaniago, M., Restiani, R., & Novita, L. (2016). Characteristics of 22 CASSAVA (Manihot esculenta Crantz) genotypes from Riau Province, Indonesia. Sabrao Journal of Breeding and Genetics, 48(2), 110-119.

• Samat, N. M. A. A., Ahmad, S., Awang, Y., Bakar, R. A. H., & Hakiman, M. (2020). Alterations in herbage yield, antioxidant activities, phytochemical contents, and bioactive compounds of Sabah snake grass (Clinacanthus Nutans L.) with regards to harvesting age and harvesting frequency. Molecules, 25(12), 1-16. https://doi.org/10.3390/molecules25122833

• Ubwa, S. T., Otache, M. A., Igbum, G. O., & Shambe, T. (2015). Determination of cyanide content in three sweet cassava cultivars in three local government areas of Benue State, Nigeria. Food and Nutrition Sciences, 06(12), 1078-1085. https://doi.org/10.4236/fns.2015.612112

• Umuhozariho, M. G., Shayo N, B., Msuya, J. M., & Sallah P., Y. K. (2014). Cyanide and selected nutrients content of different preparations of leaves from three cassava species. African Journal of Food Science, 8(3), 122-129. https://doi.org/10.5897/ajfs2013.1100

• Zitnak, A. (1973). Chronic cassava toxicity. In B. Nestel & R. MacIntyre (Eds.), Proceedings of an interdisciplinary workshop (pp. 89-96). International Development Research Centre.