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Home / Regular Issue / JST Vol. 47 (1) Feb. 2024 / JTAS-2789-2023


Effect of Effective Microorganisms in Fermentation of Rice Husk and Anchovy Head Using Lab-scale Treatment

Razifah Mohd Razali, Nur Zayani Murshidi, Mohd Sabri Mohd Ghazali and Fazilah Ariffin

Pertanika Journal of Science & Technology, Volume 47, Issue 1, February 2024


Keywords: Anchovy head, effective microorganisms, fermentation, rice husk

Published on: 23 Febuary 2024

The rice husk and anchovy filet industry is one of the world’s most successful industries and generates many biowastes with valuable bioproducts. Combining effective microorganisms (EM) with anchovy head (AH) and rice husk (RH) will produce a beneficial fertilizer and make nutrients more valuable to the plant. Thus, this study aims to determine the most effective anchovy head and rice husk treatment ratio for agricultural applications to reduce waste disposal from anchovy heads and rice husks worldwide, which may cause environmental problems. In this study, rice husk and anchovy head were fermented at five different ratios of treatment weight for 25 days: Treatment A with 100% AH, treatment B with 100% RH, treatment C with 50% AH:50% RH, treatment D with 70% AH:30% RH, and treatment E with 30% AH:70% RH. The pH, temperature, and number of colonies of every treatment were measured every 0, 5, 10, 15, 20, and 25 days. This study shows that treatment D with a ratio of 70% AH: 30% RH is the most suitable based on pH range within 6–8.5, consistently increasing temperature and the highest number of colonies to be applied to plants. The ratio of rice husk and anchovy head of treatment D in this study can be used further by other researchers to discover their potential, especially for the agricultural industry.

  • Bamdad, H., Papari, S., Lazarovits, G., & Berruti, F. (2022). Soil amendments for sustainable agriculture: Microbial organic fertilizers. Soil Use and Management, 38, 94-120.

  • Chindaprasirt, P., Jaturapitakkul, C., & Sinsiri, T. (2007). Effect of fly ash fineness on microstructure of blended cement paste. Construction and Building Materials, 21(7), 1534-1541.

  • Febria, F. A., & Rahayu, S. (2021). Development of local microorganism from organic waste as an alternative product for EM4. International Journal of Progressive Sciences and Technologies, 30(1), 98-105.

  • Firdaus, R. B. R., Tan, M. L., Rahmat, S. R., & Gunaratne, M. S. (2020). Paddy, rice and food security in Malaysia: A review of climate change impacts. Cogent Social Sciences, 6(1), 1818373.

  • Jusoh, M. L. C., Manaf, L. A., & Latiff, P. A. (2013). Composting of rice straw with effective microorganisms (EM) and its influence on compost quality. Iranian Journal of Environmental Health Science and Engineering, 10, 17.

  • Karki, G. (2020). Biofertilizer - Advantages, types, methods of application and disadvantages. Online Biology Notes.

  • Kornievskaya, E., Kurovsky, A., Babenko, A., Petrochenko, K., & Sechko, O. (2020). Microbial structure of nitrogen utilizers in Populus nigra L. compost and vermicompost. In IOP Conference Series: Earth and Environmental Science (Vol. 433, No. 1, p. 012001). IOP Publishing.

  • Milla, O. V., Rivera, E. B., Huang, W.-J., Chien, C.-C., & Wang, Y.-M. (2013). Agronomic properties and characterization of rice husk and wood biochars and their effect on the growth of water spinach in a field test. Journal of Soil Science and Plant Nutrition, 13(2), 251-266.

  • Molina-Favero, C., Creus, C. M., Simontacchi, M., Puntarulo, S., & Lamattina, L. (2008). Aerobic nitric oxide production by Azospirillum brasilense Sp245 and its influence on root architecture in tomato. Molecular Plant-Microbe Interactions, 21(7), 1001-1009.

  • Paone, E., Fazzino, F., Pizzone, D. M., Scurria, A., Pagliaro, M., Ciriminna, R., & Calabrò, P. S. (2021). Towards the anchovy biorefinery: Biogas production from anchovy processing waste after fish oil extraction with biobased limonene. Sustainability, 13(5), 2428.

  • Pode, R. (2016). Potential applications of rice husk ash waste from rice husk biomass power plant. Renewable and Sustainable Energy Reviews, 53, 1468–1485.

  • Ramli, N. H., Badrul Hisham, N. E., & Baharulrazi, N. (2023). The effectiveness of rice husk ash as additive in palm oil-based compost in enhancing the nitrogen uptake by Brassica oleracea var. alboglabra L. (Chinese kale) plant. Pertanika Journal of Tropical Agricultural Science, 46(1), 315-328.

  • Romero-Perdomo, F., Camelo-Rusinque, M., Criollo-Campos, P., & Bonilla-Buitrago, R. (2015). Effect of temperature and pH on the biomass production of Azospirillum brasilense C16 isolated from Guinea grass. Pastos Y Forraje, 38(3), 231-233.

  • Sieuwerts, S., De Bok, F. A. M., Mols, E., De Vos, W. M., & Van Hylckama Vlieg, J. E. T. (2008). A simple and fast method for determining colony forming units. Letters in Applied Microbiology, 47(4), 275-278.

  • Stoffella, P. J., & Kahn, B. A. (Eds.). (2001). Compost utilization in horticultural cropping system (1st ed.). CRC Press.

  • Talaat, N. B. (2019). Effective microorganisms: An innovative tool for inducing common bean (Phaseolus vulgaris L.) salt-tolerance by regulating photosynthetic rate and endogenous phytohormones production. Sciencetia Horticulturae, 250, 254-265.

  • Unggang, J. A. F., Bakar, M. N., & Khair, A. B. A. (2023). The potential of several wild invasive fish species as fish-based organic fertilizers on the growth of two common vegetables in Malaysia. Sains Malaysiana, 52(1), 71-81.

  • Wong, W. S., Tan, S. N., Ge, L., Chen, X., & Yong, J. W. H. (2015). The importance of phytohormones and microbes in biofertilizers. In D. Maheswari (Ed.), Bacterial metabolites in sustainable agroecosystem (pp. 105-158). Springer.

  • Yusof, M. S. M., Hajini, M. H., & Yusof, N. Y. (2022). Potential of fly larva and anchovy process waste powder as protein source in tilapia fish pellet. Politeknik and Kolej Komuniti Journal of Social Sciences and Humanities, 7(1), 49-55.

  • Zulfiqar, F., Navarro, M., Ashraf, M., Akram, N. A., & Munné-Bosch, S. (2019). Nanofertilizer use for sustainable agriculture: Advantages and limitations. Plant Science, 289, 110270.

ISSN 0128-7680

e-ISSN 2231-8526

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